Tibial dyschodroplasia (TD) is a most common pathological condition in many avian species that is characterized by failure of growth plate (GP) modeling that leads to the persistence of avascular lesion in the GP.
Tetramethylpyrazine (
TMP) is widely used to treat neurovascular disorders and
pulmonary hypertension, but no report is available about promoting effect of
TMP against TD. Therefore, a total of 210 broiler chicks were equally divided into three groups; Control, TD and
TMP. During the experiment mortality rate, chicken performance indicators (daily weight, average daily feed intake, average daily
weight gain and feed conversion ratio), tibia bone indicators (weight, length, width of tibial and the size of GP) in addition to gene expression of HIF-1α and
VEGF were examined. The results showed that
TMP administration restore the GP width, increase growth performance, and mitigated the lameness in broiler chickens. The expression of HIF-1α and
VEGF increased significantly in TD affected
thiram induced chicks. Whereas,
TMP treatment down-regulated HIF-1α and
VEGF genes and
proteins expressions. The present study demonstrates that the
TMP plays an important role in angiogenesis during the impairment and recovery of GP in TD via regulation of the HIF-1α/
VEGF signaling pathway in chickens.