Expression pattern of PD-L1 and PD-L2 in classical Hodgkin lymphoma, primary mediastinal large B-cell lymphoma, and gray zone lymphoma.

Abstract

OBJECTIVES:

We aimed at investigating the relationship between classical Hodgkin lymphoma (cHL), primary mediastinal large B-cell lymphoma (PMBL), and gray zone lymphoma (GZL) with intermediate characteristics between cHL and PMBL, from the perspective of the aberration in programed cell death 1 and the programed death ligands (PDLs) network.

METHODS:

We explored the expression levels of PDLs and chromosomal anomalies in 67 cases: 34 cases with cHL, 20 with PMBL, and 13 with GZL, using immunohistochemical analyses and Fluorescence In Situ Hybridization (FISH).

RESULTS:

Twenty-one cHL (62%), 3 PMBL (15%), and 6 GZL (46%) cases showed staining to PD-L1 antibodies in more than 70% of tumor cells. Two cHL (6%), 10 PMBL (50%), and 3 GZL (23%) cases were not stained by PD-L1 antibodies. Patients over 40 years old manifest more frequent expression of PD-L1 in cHL. Proportion of tumors stained by PD-L2 antibody was increased in PMBL. FISH analyses with a PD-L1/PD-L2 probe detected 5 amplification, 1 gain, and 7 polysomy cases in cHL, 1 amplification and 1 polysomy case in GZL, and amplification in 1 PMBL case.

CONCLUSION:

We identified increased staining of PD-L1 in cHL and that of PD-L2 in PMBL. GZL had a pattern similar to that of cHL.

• Authors: Yosuke Tanaka, Akiko Miyagi Maeshima, Junko Nomoto, Shinichi Makita, Suguru Fukuhara, Wataru Munakata, Dai Maruyama, Kensei Tobinai, Yukio Kobayashi
• Journal: European journal of haematology (Eur J Haematol) Vol. 100 Issue 5 Pg. 511-517 (May 2018) ISSN: 1600-0609 [Electronic] England
• PMID: 29377256 (Publication Type: Journal Article)
• Copyright: © 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Chemical References

• B7-H1 Antigen
• Biomarkers, Tumor
• CD274 protein, human
• PDCD1LG2 protein, human
• Programmed Cell Death 1 Ligand 2 Protein

Topics

• Adolescent
• Adult
• Aged
• B7-H1 Antigen (genetics, metabolism)
• Biomarkers, Tumor
• Biopsy
• Female
• Gene Expression
• Hodgkin Disease (diagnosis, genetics, mortality)
• Humans
• Immunohistochemistry
• In Situ Hybridization, Fluorescence
• Lymphoma, Large B-Cell, Diffuse (diagnosis, genetics, mortality)
• Male
• Mediastinal Neoplasms (diagnosis, genetics, mortality)
• Middle Aged
• Neoplasm Staging
• Prognosis
• Programmed Cell Death 1 Ligand 2 Protein (genetics, metabolism)
• Young Adult