Effects of Wy-18,251 (3-p-chlorophenyl)thiazolo[3,2-a]benzimidazole- 2-acetic acid), levamisole and indomethacin on the generation of murine T suppressor cells in vitro.

Abstract

In vitro culture of normal BALB/c spleen cells with staphylococcal enterotoxin B (SEB) activates antigen non-specific suppressor T cells (Ts) which can be assayed by their ability to suppress antibody production in a plaque assay. Addition of the experimental immunomodulatory drug Wy-18,251 (10-100 microM) to cultures of spleen cells plus SEB significantly increased Ts activity relative to cultures without the drug. Similar results were obtained with levamisole, but, in contrast, indomethacin (0.1-10 microM) inhibited SEB-induced suppressor cell activity. The ability of Wy-18,251 to augment Ts activity could be therapeutically useful in the treatment of those autoimmune diseases, such as rheumatoid arthritis and systemic lupus erythematosus, in which hyperactive B cell function is a characteristic feature.

• Authors: C M Rogers, T J Rogers, S C Gilman
• Journal: Journal of immunopharmacology (J Immunopharmacol) Vol. 7 Issue 4 Pg. 479-88 ( 1985) ISSN: 0163-0571 [Print] United States
• PMID: 2935580 (Publication Type: Journal Article)

Chemical References

• Benzimidazoles
• Enterotoxins
• Levamisole
• enterotoxin B, staphylococcal
• tilomisole
• Indomethacin

Topics

• Animals
• Benzimidazoles (pharmacology)
• Enterotoxins (immunology)
• In Vitro Techniques
• Indomethacin (pharmacology)
• Levamisole (pharmacology)
• Male
• Mice
• Mice, Inbred BALB C
• Spleen (cytology, immunology)
• T-Lymphocytes, Regulatory (drug effects, immunology)