NPTX1 inhibits colon cancer cell proliferation through down-regulating cyclin A2 and CDK2 expression.

Abstract

Colon cancer is the third most common malignancy and one of the leading causes of cancer-associated mortality worldwide. Neuronal pentraxin 1 (NPTX1) is associated with tumor progression in some types of tumors. However, its expression and role in colon cancer has not been yet reported. Here we observed that NPTX1 was down-regulated in colon cancer. Additionally, we explored the functional significance of NPTX1 in colon cancer. We found that over-expression of NPTX1 inhibited colon cancer cell growth by performing MTT, colony formation, Edu corporation assays, and cell cycle analysis. In vivo mouse experiments also confirmed the anti-proliferative role of NPTX1 in colon cancer. Further mechanistic study showed that over-expression of NPTX1 inhibited the expression of cyclin A2 and CDK2 in colon cancer cells, thereby regulating the Rb-E2F signaling. In summary, these findings reveal that NPTX1 suppress the colon cancer cell growth and might serve as a useful potential target for treatment of colon cancer.

• Authors: Xiaofeng Peng, Kangming Pan, Wenli Zhao, Jianzhu Zhang, Shicheng Yuan, Xiang Wen, Wenquan Zhou, Zhijin Yu
• Journal: Cell biology international (Cell Biol Int) Vol. 42 Issue 5 Pg. 589-597 (May 2018) ISSN: 1095-8355 [Electronic] England
• PMID: 29345391 (Publication Type: Journal Article)
• Copyright: © 2018 International Federation for Cell Biology.

Chemical References

• CCNA2 protein, human
• Cyclin A2
• Nerve Tissue Proteins
• neuronal pentraxin
• C-Reactive Protein
• CDK2 protein, human
• Cyclin-Dependent Kinase 2

Topics

• Animals
• C-Reactive Protein (metabolism, physiology)
• Cell Line, Tumor
• Cell Proliferation
• Colonic Neoplasms (metabolism, pathology)
• Cyclin A2 (metabolism)
• Cyclin-Dependent Kinase 2 (metabolism)
• Down-Regulation
• Humans
• Mice
• Mice, Nude
• Nerve Tissue Proteins (metabolism, physiology)
• Xenograft Model Antitumor Assays