[Immunomodulating and antitumor activity of polysaccharides of plant origin].

Abstract

The effect of the polysaccharides tagetan, palustran, and zymosan injections to BALB/c mice on the formation of cytotoxic T-lymphocytes in allogenic mixed culture has been studied. Lymphocytes from a 5-day-old mixed culture were characterized by the greatest cytotoxic activity, with spleenocytes serving as reacting cells in mice injected 12-50 mg/kg of tagetan, 20 mg/kg of zymosan and 10-100 mg/kg of palustran, 7, 3 and 5 days, respectively, before the beginning of allogeneic stimulation in vitro. To determine correlation between immunostimulating and antitumor activity 25 mg/kg of tagetan every 14 days, 20 mg/kg of zymosan every 6 days, and 10 mg/kg of palustran every 6 days, were injected to BALB/c and BALB/c nu/nu mice prior to and following subcutaneous implantation of Crocker sarcoma cells. By the end of the first month of immunotherapy the tumour size in mice on tagetan or zymosan was 2-2,5 times smaller than in control animals. Tumour growth inhibition with palustran was about 30%. Polysaccharide administration to BALB/c nu/nu mice was not accompanied by tumour growth inhibition. The data obtained suggest that inhibition of Crocker sarcoma growth in mice injected the above polysaccharides is mediated by stimulation of antitumour T-cell activity.

• Authors: A V Sergeev, E S Revazova, S I Denisova, O V Kalatskaia, A N Rytenko
• Journal: Biulleten' eksperimental'noi biologii i meditsiny (Biull Eksp Biol Med) Vol. 100 Issue 12 Pg. 741-3 (Dec 1985) ISSN: 0365-9615 [Print] Russia (Federation)
• Vernacular Title: Immunomoduliruiushchaia i protivoopukholevaia aktivnost' polisakharidov rastitel'nogo proiskhozhdeniia.
• PMID: 2934103 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Adjuvants, Immunologic
• Antineoplastic Agents, Phytogenic
• Polysaccharides
• palustran
• tagetan

Topics

• Adjuvants, Immunologic (pharmacology, therapeutic use)
• Animals
• Antineoplastic Agents, Phytogenic (pharmacology, therapeutic use)
• Cytotoxicity Tests, Immunologic
• Dose-Response Relationship, Drug
• Drug Evaluation, Preclinical
• L Cells (drug effects)
• Lymphocyte Culture Test, Mixed
• Male
• Mice
• Mice, Inbred BALB C
• Mice, Inbred C3H
• Neoplasm Transplantation
• Phytotherapy
• Plants, Medicinal
• Polysaccharides (pharmacology, therapeutic use)
• Sarcoma 180 (drug therapy)
• T-Lymphocytes, Cytotoxic (drug effects)
• Time Factors