Long non-coding RNA HOTAIR acts as a competing endogenous RNA to promote glioma progression by sponging miR-126-5p.

Abstract

LncRNA HOX transcript antisense intergenic RNA (HOTAIR) has been shown to play prominent roles in tumorigenesis. However, its precise molecular mechanism in glioma has not been completely elucidated. In this study, we found that HOTAIR was aberrantly up-regulated in glioma tissues and was negatively correlated with miR-126-5p expression. Next, we determined that HOTAIR promote glioma progression by sponging miR-126-5p. Subsequently, glutaminase (GLS) was confirmed to be a direct target of miR-126-5p using bioinformatics software and a luciferase reporter assay. Moreover, HOTAIR could modulate GLS expression by functioning as a competing endogenous RNA (ceRNA) for miR-126-5p. Taken together, our study clarified that the HOTAIR/miR-126/GLS pathway is involved in glioma progression and may potentially serve as a target for glioma therapy.

• Authors: Liang Liu, Sitong Cui, Teng Wan, Xiaojian Li, Wei Tian, Rui Zhang, Liangsheng Luo, Yan Shi
• Journal: Journal of cellular physiology (J Cell Physiol) Vol. 233 Issue 9 Pg. 6822-6831 (09 2018) ISSN: 1097-4652 [Electronic] United States
• PMID: 29319172 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2018 Wiley Periodicals, Inc.

Chemical References

• HOTAIR long untranslated RNA, human
• MIRN126 microRNA, human
• MicroRNAs
• RNA, Long Noncoding
• Glutaminase

Topics

• Animals
• Carcinogenesis (genetics, pathology)
• Cell Line, Tumor
• Disease Progression
• Gene Expression Regulation, Neoplastic (genetics)
• Glioma (genetics, pathology)
• Glutaminase (genetics)
• Humans
• Male
• Mice
• Mice, Nude
• MicroRNAs (genetics)
• RNA, Long Noncoding (genetics)
• Up-Regulation (genetics)