Abstract |
Kodak Q-switch II dye has recently proven to be an effective biostimulative agent on normal human fibroblast cultures. The potential for this dye as a new chemosensitizing agent for the treatment of connective tissue diseases and wound healing with the Nd:YAG laser was examined. Two normal fibroblast cell lines were first sensitized to a nontoxic dose of Q-switch II dye, then subjected to treatment with an Nd:YAG laser at 1,060 nm, with varying levels of energy and temperatures determined by a reproducible method of dosimetry. The results indicate that Q-switch II dye at nontoxic doses of 0.1 micrograms/ml enhances the cytotoxic effects of the Nd:YAG laser at temperatures as low as 36 degrees C. Furthermore, at physiological temperature ranges as low as 24 degrees C to 34 degrees C, cell duplication was inhibited, but cell viability was not affected. Similar results were not observed when fibroblast cultures were treated with the laser alone. These observations suggest that Q-switch II dye is an effective chemosensitizing agent for the Nd:YAG laser and could potentially be used to reduce collagen deposits in conditions such as keloids and hypertrophic scars.
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Authors | D J Castro, R E Saxton, H R Fetterman, D J Castro, P H Ward |
Journal | The Laryngoscope
(Laryngoscope)
Vol. 99
Issue 4
Pg. 421-8
(Apr 1989)
ISSN: 0023-852X [Print] United States |
PMID | 2927219
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Benzopyrans
- Fluorescent Dyes
- Q-switch II dye
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Topics |
- Benzopyrans
(pharmacology)
- Cell Survival
- Cells, Cultured
- Fibroblasts
(cytology)
- Fluorescent Dyes
(pharmacology)
- Humans
- In Vitro Techniques
- Laser Therapy
- Photochemotherapy
(methods)
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