Anti-proliferative activity of a purified polysaccharide isolated from the basidiomycete fungus Paxillus involutus.

Abstract

A growth-inhibitory polysaccharide (GIPinv) was purified using size-exclusion and ion-exchange chromatography from the fourth sodium hydroxide extraction step of a fungus found in British Columbia. The fungus was genetically identified as a member of the Paxillus involutus complex. GIPinv has an average molecular weight of 229kDa and is a heteroglycan composed of glucose (65.9%), galactose (20.8%), mannose (7.8%), fucose (3.2%) and xylose (2.3%). GC-MS methylation analysis suggests that GIPinv has mixed linkages in the backbone containing (1→6)-Gal (25.5%), (1→4)-Glc (18.3%), (1→6)-Glc (8.3%), (1→3)-Glc (5.3%) and (1→2)-Xyl (4.5%). GIPinv has branching points at (1→2, 6)-Man (8.6%) and (1→3, 6)-Man (4.9%) having unsubstituted fucose (8.3%) and glucose (16.3%) as terminal sugars. GIPinv had growth-inhibitory activity against several cancer cell lines and triggered apoptosis. GIPinv should be further explored as a potential anti-cancer agent and a unique polysaccharide.

• Authors: Ankush Barad, Sebastian Mackedenski, Wai Ming Li, Xiao Jie Li, Bryan Chu Chwen Lim, Faran Rashid, Linda E Tackaberry, Hugues B Massicotte, Keith N Egger, Kerry Reimer, Peter C K Cheung, Chow H Lee
• Journal: Carbohydrate polymers (Carbohydr Polym) Vol. 181 Pg. 923-930 (Feb 01 2018) ISSN: 1879-1344 [Electronic] England
• PMID: 29254055 (Publication Type: Journal Article)
• Copyright: Copyright © 2017 Elsevier Ltd. All rights reserved.

Chemical References

• Complex Mixtures
• Monosaccharides
• Polysaccharides

Topics

• Apoptosis (drug effects)
• Basidiomycota (chemistry)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Chemical Fractionation
• Complex Mixtures
• Gas Chromatography-Mass Spectrometry
• Humans
• Monosaccharides (analysis)
• Phylogeny
• Polysaccharides (isolation & purification, pharmacology)