Identification of antigenic peptides from novel renal cancer stem-like cell antigen, DNAJB8.

Abstract

OBJECTIVES:

To identify antigenic peptides of cancer stem-like cells (CSCs) antigen, DNAJB8, and establish a mouse CSCs-targeting immunotherapy model.

MATERIALS AND METHODS:

To induce DNAJB8-specific immune reaction, we stimulated human CD8+ lymphocytes with antigen-presenting cells pulsed with a cocktail of three candidate HLA-A*24:02 restricted peptides and assessed peptide specific human cytotoxic T lymphocytes (CTLs) induction. One of the antigenic peptides showed identical amino acid sequence as corresponding mouse DNAJB8. We evaluated CTL induction with the peptide immunization in mouse model.

RESULTS:

We confirmed peptide-specific interferon-γ secretions and cytotoxic activities of induced human CTLs. In vivo immunization with the peptide to mice, peptide-specific CTL response could be observed in mouse CD8+ T cells. Furthermore, immunization with the peptide showed significant anti-tumor effects compared with negative controls.

CONCLUSION:

DNAJB8-derived peptide is a novel candidate for CSCs-targeting immunotherapy, and mouse models can be used to evaluate CSCs-targeting immunotherapy.

• Authors: Satoshi Nishizawa, Yoshihiko Hirohashi, Hiroki Kusumoto, Takahito Wakamiya, Takashi Iguchi, Shimpei Yamashita, Akinori Iba, Kazuro Kikkawa, Yasuo Kohjimoto, Toshihiko Torigoe, Isao Hara
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 494 Issue 3-4 Pg. 693-699 (12 16 2017) ISSN: 1090-2104 [Electronic] United States
• PMID: 29107688 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2017 Elsevier Inc. All rights reserved.

Chemical References

• Antigens, Neoplasm
• HLA-A*24:02 antigen
• HLA-A24 Antigen
• Peptides

Topics

• Animals
• Antigens, Neoplasm (immunology)
• Apoptosis (drug effects, immunology)
• Carcinoma, Renal Cell (drug therapy, immunology, pathology)
• Cell Line, Tumor
• Drug Discovery (methods)
• Epitope Mapping (methods)
• Female
• HLA-A24 Antigen
• Kidney Neoplasms (drug therapy, immunology, pathology)
• Mice
• Mice, Inbred BALB C
• Neoplastic Stem Cells (immunology)
• Peptides (administration & dosage, immunology)