Effects of lys-beta-urogastrone in vivo.

Abstract

Lys-beta-urogastrone, an analogue of human beta-urogastrone with an additional N-terminal lysine, was shown to have similar effects in mice and sheep to mouse epidermal growth factor (mEGF). Lys-beta-urogastrone in doses of 0.18-3.24 micrograms g-1 body weight caused both precocious separation of eyelids and eruption of incisors in neonatal mice. In 17 sheep, intravenous infusion of the urogastrone analogue over c. 24 h led, towards the end of infusion, to erythema of the muzzle, caused reductions in voluntary food intake (with doses greater than or equal to 50 micrograms kg-1) and generally easier manual harvesting of the fleece (with infusions greater than or equal to 81 micrograms kg-1), with spontaneous shedding of the fleece (c. 14 days after infusions of greater than or equal to 116 micrograms kg-1). In five sheep infusions of 25, 38, 50, 83 and 118 micrograms kg-1 fleece-free body weight, plasma concentrations of lys-beta-urogastrone were near maximal 20 h after the infusions started and were, respectively, 1.1, 1.7, 5.5, 18 and 79 micrograms l-1 plasma. Plasma concentrations of gastrin, somatostatin and pancreatic polypeptide were determined in these five sheep. Plasma gastrin rose sixfold by the end of infusions of 25 micrograms kg-1 of the urogastrone analogue, and tenfold with the higher doses of infusion. Although plasma somatostatin concentrations were variable, a consistent trend was observed; lower levels were apparent during the lys-beta-urogastrone infusions. There was no discernible trend in pancreatic polypeptide concentrations.

• Authors: A J Campbell, S S Adams, M W Davey, D A Titchen
• Journal: Australian journal of biological sciences (Aust J Biol Sci) Vol. 41 Issue 4 Pg. 463-74 ( 1988) ISSN: 0004-9417 [Print] Australia
• PMID: 2908424 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Gastrins
• urogastrone, Lys-beta-
• Somatostatin
• Pancreatic Polypeptide
• Epidermal Growth Factor

Topics

• Animals
• Animals, Suckling (growth & development)
• Eating (drug effects)
• Epidermal Growth Factor (administration & dosage, pharmacokinetics, pharmacology)
• Erythema (chemically induced)
• Eyelids (drug effects, growth & development)
• Female
• Gastrins (blood)
• Incisor (drug effects, growth & development)
• Male
• Mice
• Pancreatic Polypeptide (blood)
• Sheep (physiology)
• Somatostatin (blood)
• Wool (drug effects, growth & development)