β-Glucans in food modify colonic microflora by inducing antimicrobial protein, calprotectin, in a Dectin-1-induced-IL-17F-dependent manner.

Abstract

Dectin-1 (gene symbol: Clec7a) is a receptor for β-glucans that play an important role for the host defense against fungi. Recently, we showed that Clec7a-/- mice are resistant against dextran sodium sulfate (DSS)-induced colitis because of regulatory T-cell population expansion in the colon. The regulatory T-cell expansion is caused by expansion of commensal Lactobacillus murinus whose growth is suppressed by an antimicrobial protein, calprotectin S100A8/A9. In this report, we showed that S100A8 was mainly produced by mouse colonic epithelial cells. S100A8 was not induced directly by Dectin-1 but by Dectin-1-induced cytokines, especially interleukin-17F (IL-17F), that were produced by several types of innate immune cells including CD11c+/CD11b+ myeloid cells in colonic lamina propria. S100A8/A9 heterodimer preferentially suppressed the growth of L. murinus that was increased in both Clec7a-/- and Il17f-/- mice. Furthermore, similar expansion of L. murinus and DSS-colitis resistance were observed in mice fed with β-glucan-free food. These observations suggest that food-derived β-glucans control the specific commensal microbiota via the Dectin-1-IL-17F-calprotectin axis to maintain the intestinal homeostasis.

• Authors: T Kamiya, C Tang, M Kadoki, K Oshima, M Hattori, S Saijo, Y Adachi, N Ohno, Y Iwakura
• Journal: Mucosal immunology (Mucosal Immunol) Vol. 11 Issue 3 Pg. 763-773 (05 2018) ISSN: 1935-3456 [Electronic] United States
• PMID: 29068000 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Infective Agents
• Calgranulin A
• Interleukin-17
• Lectins, C-Type
• Leukocyte L1 Antigen Complex
• beta-Glucans
• dectin 1

Topics

• Animals
• Anti-Infective Agents (metabolism)
• Calgranulin A (metabolism)
• Colitis (chemically induced, genetics, immunology)
• Colon (immunology)
• Food
• Gastrointestinal Microbiome (physiology)
• Host Microbial Interactions
• Interleukin-17 (genetics, metabolism)
• Intestinal Mucosa (physiology)
• Lactobacillus (physiology)
• Lectins, C-Type (genetics, metabolism)
• Leukocyte L1 Antigen Complex (genetics, metabolism)
• Mice
• Mice, Inbred C57BL
• Mice, Knockout
• Myeloid Cells (physiology)
• beta-Glucans (administration & dosage, metabolism)