Lack of D-amino-acid oxidase activity causes a specific renal aminoaciduria in the mouse.

Abstract

Thin-layer chromatography and amino acid analysis showed that urine of mutant ddY/DAO- mice lacking D-amino-acid oxidase activity contained more serine, proline, alanine and methionine than that of normal ddY/DAO+ mice. Among these four, an increase in alanine was conspicuous. However, the urinary levels of 11 other amino acids and glucose were not different between the ddY/DAO- and ddY/DAO+ mice. Amino acid analysis showed that the plasma levels of serine, proline and methionine were not elevated in the ddY/DAO- mice, though a slight increase in alanine was observed. Genetic crosses showed that aminoaciduria and lack of D-amino-acid oxidase activity were concomitantly transmitted as a set through generations. These results indicated that the lack of enzyme activity caused a specific renal aminoaciduria. Whether this enzyme merely diminishes the D-amino acid load presented for reabsorption, or actually participates catalytically in the reabsorption process, remains undetermined.

• Authors: R Konno, K Isobe, A Niwa, Y Yasumura
• Journal: Biochimica et biophysica acta (Biochim Biophys Acta) Vol. 967 Issue 3 Pg. 382-90 (Dec 15 1988) ISSN: 0006-3002 [Print] Netherlands
• PMID: 2904279 (Publication Type: Journal Article)

Chemical References

• Amino Acids
• D-Amino-Acid Oxidase

Topics

• Amino Acids (blood, urine)
• Animals
• Crosses, Genetic
• D-Amino-Acid Oxidase (deficiency)
• Female
• Male
• Mice
• Mice, Inbred Strains
• Mice, Mutant Strains
• Renal Aminoacidurias (urine)