Antioxidant, antibacterial and antiproliferative activities of pumpkin (cucurbit) peel and puree extracts - an in vitro study.

Abstract

Natural resources right from the beginning of the human civilization has paved the way to human being to combat different challenges. The big challenge was to safe the human being from diseases and shortage of food. Plants helped the man in both areas very efficiently. No doubt when plants are used as food actually we are also taking lot of compounds of medicinal values in an excellent combination which naturally reduce the risk of diseases. Extraction and purification of several medicinally important compounds also gave the way to develop pharmaceutical industry in addition to its own therapeutic effects against different lethal diseases. Pumpkin is one of the several medicinal important vegetables used in different way on the behalf of its admirable power to combat different diseases. Antioxidant and biological studies showed very important results. A good coherence was found among extraction yield (10.52 to 18.45%), total phenolics (1.13 to 6.78 mg GAE/100g), total flavonoids (0.23 to 0.72mg CE/100g) and antioxidant potential (≻70%). Antibacterial assays of peel and puree extracts advocated good potential to stop the growth and division of pathogenic bacteria. Further biological activity study was carried out using MDBK cancer cell line. The growth inhibitory effect on cancer cell line using MTT assay showed methanol extracts of peel and puree both remained efficient to inhibit growth (≻35%) and cell division of cancer cells. Our results showed that extracts of pumpkin puree and its waste, peel, may be utilize to prepare functional food against pathogenic born diseases and most active compounds may also be extracted, concentrated and converted into tablets or suspension form for therapeutic purposes.

• Authors: Muhammad Asif, Syed Ali Raza Naqvi, Tauqir A Sherazi, Matloob Ahmad, Ameer Fawad Zahoor, Sohail Anjum Shahzad, Zaib Hussain, Hassan Mahmood, Nasir Mahmood
• Journal: Pakistan journal of pharmaceutical sciences (Pak J Pharm Sci) Vol. 30 Issue 4 Pg. 1327-1334 (Jul 2017) ISSN: 1011-601X [Print] Pakistan
• PMID: 29039333 (Publication Type: Journal Article)

Chemical References

• Anti-Bacterial Agents
• Antioxidants
• Flavonoids
• Free Radical Scavengers
• Phenols
• Plant Extracts

Topics

• Anti-Bacterial Agents (chemistry, pharmacology)
• Antioxidants (chemistry, pharmacology)
• Cell Division (drug effects)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Cucurbita (chemistry)
• Flavonoids (analysis)
• Free Radical Scavengers (chemistry, pharmacology)
• Humans
• Inhibitory Concentration 50
• Microbial Sensitivity Tests
• Phenols (analysis)
• Plant Extracts (chemistry, pharmacology)