Measurement of Murine Single-Kidney Glomerular Filtration Rate Using Dynamic Contrast-Enhanced MRI.

Abstract

PURPOSE:

To develop and validate a method for measuring murine single-kidney glomerular filtration rate (GFR) using dynamic contrast-enhanced MRI (DCE-MRI).

METHODS:

This prospective study was approved by the Institutional Animal Care and Use Committee. A fast longitudinal relaxation time (T1 ) measurement method was implemented to capture gadolinium dynamics (1 s/scan), and a modified two-compartment model was developed to quantify GFR as well as renal perfusion using 16.4T MRI in mice 2 weeks after unilateral renal artery stenosis (RAS, n = 6) or sham (n = 8) surgeries. This approach was validated by comparing model-derived GFR and perfusion to those obtained by fluorescein isothiocyanante (FITC)-inulin clearance and arterial spin labeling (ASL), respectively, using the Pearson's and Spearman's rank correlations and Bland-Altman analysis.

RESULTS:

The compartmental model provided a good fitting to measured gadolinium dynamics in both normal and RAS kidneys. The proposed DCE-MRI method offered assessment of single-kidney GFR and perfusion, comparable to the FITC-inulin clearance (Pearson's correlation coefficient r = 0.95 and Spearman's correlation coefficient ρ = 0.94, P < 0.0001, and mean difference -7.0 ± 11.0 μL/min) and ASL (r = 0.92 and ρ = 0.84, P < 0.0001, and mean difference 4.4 ± 66.1 mL/100 g/min) methods.

CONCLUSION:

The proposed DCE-MRI method may be useful for reliable noninvasive measurements of single-kidney GFR and perfusion in mice. Magn Reson Med 79:2935-2943, 2018. © 2017 International Society for Magnetic Resonance in Medicine.

• Authors: Kai Jiang, Hui Tang, Prasanna K Mishra, Slobodan I Macura, Lilach O Lerman
• Journal: Magnetic resonance in medicine (Magn Reson Med) Vol. 79 Issue 6 Pg. 2935-2943 (06 2018) ISSN: 1522-2594 [Electronic] United States
• PMID: 29034514 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
• Copyright: © 2017 International Society for Magnetic Resonance in Medicine.

Chemical References

• Contrast Media
• Spin Labels
• Inulin
• Fluorescein-5-isothiocyanate

Topics

• Animals
• Arteries (pathology)
• Body Weight
• Contrast Media (chemistry)
• Fluorescein-5-isothiocyanate (chemistry)
• Glomerular Filtration Rate
• Image Interpretation, Computer-Assisted (methods)
• Image Processing, Computer-Assisted
• Inulin (chemistry)
• Kidney (diagnostic imaging)
• Kidney Function Tests (methods)
• Magnetic Resonance Imaging
• Mice
• Perfusion
• Prospective Studies
• Reproducibility of Results
• Spin Labels