[Regulation of the transcription of the tyrosine aminotransferase gene by glucocorticoids in Morris hepatoma 7777 and 8994 cells].

Abstract

Kinetics of tyrosine aminotransferase (TAT) mRNA synthesis in Morris rat hepatoma cell lines 7777 and 8994 after dexamethasone treatment (10(-7) M) was studied by molecular hybridization of the RNA with cloned fragments of TAT gene from rat liver cells. It was demonstrated that initiation of TAT gene transcription increased 20 minutes after glucocorticoid treatment. The level of TAT mRNA was not induced by dexamethasone in rat hepatoma cell line 8994. Actinomycin D prevented the deinduction of TAT by stabilization of TAT mRNA.

• Authors: V V Adler, M A Krasil'nikov, A V Polotskaia
• Journal: Molekuliarnaia biologiia (Mol Biol (Mosk)) 1988 Mar-Apr Vol. 22 Issue 2 Pg. 423-9 ISSN: 0026-8984 [Print] Russia (Federation)
• Vernacular Title: Reguliatsiia transkriptsii gena tirozinaminotransferazy gliukokortikoidami v kletkakh gepatomy Morrisa 7777 i 8994.
• PMID: 2899290 (Publication Type: English Abstract, Journal Article)

Chemical References

• Glucocorticoids
• Dactinomycin
• Tyrosine Transaminase

Topics

• Animals
• Dactinomycin (pharmacology)
• Enzyme Induction
• Genes
• Glucocorticoids (pharmacology)
• Liver Neoplasms, Experimental (enzymology, genetics)
• Rats
• Transcription, Genetic (drug effects)
• Tumor Cells, Cultured (drug effects, enzymology)
• Tyrosine Transaminase (biosynthesis, genetics)