Stachydrine (Sta), a major constituent of Leonurus japonicus Houtt, has been reported to possess numerous cardioprotective effects. In this study, we evaluated the effect of Sta on pressure overload-induced
diastolic heart failure in rats and investigated the mechanisms underlying the effect. Wistar rats were randomized to transverse aortic constriction (TAC) or
sham operation. After 3 days, the rats that underwent TAC were randomized to treatment for a total of four experimental groups (n=10 each group):
sham operation, TAC only, TAC +
telmisartan (Tel), and TAC +
stachydrine (Sta). After 12 weeks, we evaluated
left ventricular hypertrophy, function, and
fibrosis by echocardiography, pressure-volume loop analysis, and histology. In addition, levels of
fibrosis-related
proteins in the heart were determined by Western blot analysis. Our results showed that Sta significantly suppressed TAC-induced
cardiac hypertrophy, and TAC-induced increases in heart
weight/body weight and heart weight/tibial length. In addition, Sta attenuated TAC-induced decreases in left ventricular ejection fraction and improved other hemodynamic parameters. Compared with the TAC only group, rats treated with Sta exhibited significant decreases in interstitial and perivascular
fibrosis, TGF-βR1
protein levels, and phosphorylation of Smad2/3; however,
protein levels of TGF-β1, TGF-βR2, and Smad4 did not differ significantly between the two groups. Taken together, our results demonstrate that Sta protects against
diastolic heart failure by attenuating myocardial
hypertrophy and
fibrosis via the TGF-β/Smad pathway.