P-fimbriae receptors in patients with chronic pyelonephritis.

Abstract

The adherence of fluorescein isothiocyanate-labeled P-fimbriated Escherichia coli to uroepithelial cells from 19 women with chronic pyelonephritis was determined with the fluorescence-activated cell sorting technique. The application of this method has made it possible to study bacterial binding to a large number of cells. Renal function was determined in all patients and the recurrences of P-fimbriated Escherichia coli bacteriuria, cystitis and acute pyelonephritis during a 3-year followup were studied. We found a significant correlation between the P-fimbriae receptor accessibility on uroepithelial cells and glomerular filtration rate (r equals -0.75, p less than 0.001). Uroepithelial cells from the patients with chronic pyelonephritis and renal insufficiency had a higher binding capacity of P-fimbriated Escherichia coli than uroepithelial cells from patients with a normal glomerular filtration rate. There was no correlation between kidney function and the availability of P-fimbriae receptors in a control group of patients with polycystic kidney disease.

• Authors: S H Jacobson, G Källenius, L E Lins, S B Svenson
• Journal: The Journal of urology (J Urol) Vol. 139 Issue 5 Pg. 900-3 (May 1988) ISSN: 0022-5347 [Print] United States
• PMID: 2896254 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Receptors, Immunologic
• pili, bacterial receptor

Topics

• Adult
• Bacterial Adhesion
• Cell Separation
• Epithelium (immunology)
• Escherichia coli (immunology)
• Escherichia coli Infections (immunology, physiopathology)
• Female
• Fimbriae, Bacterial (immunology)
• Flow Cytometry
• Glomerular Filtration Rate
• Humans
• Kidney (physiopathology)
• Middle Aged
• Pyelonephritis (immunology, physiopathology)
• Receptors, Immunologic (immunology)