IL-2 receptor(p55)/Tac-inducing factor. Purification and characterization of adult T cell leukemia-derived factor.

Abstract

Many human T cell lymphotropic virus-I (HTLV-I) transformed T cells from adult T cell leukemia (ATL) patients continuously produce a humoral factor called ATL-derived factor (ADF) which induces IL-2R/Tac expression on T and NK cells. Using gel filtration, procion red Sepharose, DEAE, and reverse phase chromatography, we have purified ADF protein to homogeneity from 15 liters of serum-free culture supernatant of an HTLV-I(+) T cell line ATL-2. Purified ADF protein had the m.w. of 14,000 by SDS-PAGE and gel filtration, and its isoelectric point is around 5.0. ADF did not react with heteroantibodies against IL-1 alpha and IL-1 beta, which have also IL-2R/Tac-inducing activity on suitable target cells. Partial N-terminal amino acid sequence of ADF is different from other cytokines such as, IFN, BSF-2, and various IL whose cDNA has been cloned. Western blot analysis using rabbit antibodies against N-terminal 10mer synthetic peptide of ADF showed that IL-1 alpha and ADF are different proteins. ADF had its IL-2R/Tac-inducing activity not only on human NK-like cell line YT, but also on HTLV-I(+) T cells, such as ED. In contrast, macrophage-derived IL-1 lacked IL-2R/Tac-inducing activity on ED cells despite their IL-2R/Tac induction on YT, indicating that ADF and IL-1 have their effect via different receptors.

• Authors: Y Tagaya, M Okada, K Sugie, T Kasahara, N Kondo, J Hamuro, K Matsushima, C A Dinarello, J Yodoi
• Journal: Journal of immunology (Baltimore, Md. : 1950) (J Immunol) Vol. 140 Issue 8 Pg. 2614-20 (Apr 15 1988) ISSN: 0022-1767 [Print] United States
• PMID: 2895791 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cytokines
• Interleukin-1
• Neoplasm Proteins
• Receptors, Immunologic
• Receptors, Interleukin-2
• adult T cell leukemia-derived factor

Topics

• Adolescent
• Amino Acid Sequence
• Antibody Specificity
• Cell Line
• Cytokines
• Deltaretrovirus Infections (analysis)
• Gene Expression Regulation (drug effects)
• Humans
• Interleukin-1 (immunology, pharmacology)
• Killer Cells, Natural (drug effects)
• Lymphocyte Activation
• Male
• Molecular Sequence Data
• Neoplasm Proteins (immunology, isolation & purification, pharmacology)
• Receptors, Immunologic (biosynthesis)
• Receptors, Interleukin-2
• T-Lymphocytes (analysis, drug effects)