Cervical cancer stem-like cells: systematic review and identification of reference genes for gene expression.

Abstract

Cervical cancer is the fourth most common cancer affecting women worldwide. Among many factors, the presence of cancer stem cells, a subpopulation of cells inside the tumor, has been associated with a worse prognosis. Considering the importance of gene expression studies to understand the biology of cervical cancer stem cells (CCSC), this work identifies stable reference genes for cervical cancer cell lines SiHa, HeLa, and ME180 as well as their respective cancer stem-like cells. A literature review was performed to identify validated reference genes currently used to normalize RT-qPCR data in cervical cancer cell lines. Then, cell lines were cultured in regular monolayer or in a condition that favors tumor sphere formation. RT-qPCR was performed using five reference genes: ACTB, B2M, GAPDH, HPRT1, and TBP. Stability was assessed to validate the selected genes as suitable reference genes. The evaluation validated B2M, GAPDH, HPRT1, and TBP in these experimental conditions. Among them, GAPDH and TBP presented the lowest variability according to the analysis by Normfinder, Bestkeeper, and ΔCq methods, being therefore the most adequate genes to normalize the combination of all samples. These results suggest that B2M, GAPDH, HPRT1, and TBP are suitable reference genes to normalize RT-qPCR data of established cervical cancer cell lines SiHa, HeLa, and ME180 as well as their derived cancer stem-like cells. Indeed, GAPDH and TBP seem to be the most convenient choices for studying gene expression in these cells in monolayers or spheres.

• Authors: Rafael P de Campos, Iago C Schultz, Paola de Andrade Mello, Samuel Davies, Manuela S Gasparin, Ana P S Bertoni, Andréia Buffon, Márcia R Wink
• Journal: Cell biology international (Cell Biol Int) Vol. 42 Issue 2 Pg. 139-152 (Feb 2018) ISSN: 1095-8355 [Electronic] England
• PMID: 28949053 (Publication Type: Journal Article, Systematic Review)
• Copyright: © 2017 International Federation for Cell Biology.

Topics

• Cell Line, Tumor
• Female
• HeLa Cells
• Humans
• Neoplastic Stem Cells (metabolism, pathology)
• Real-Time Polymerase Chain Reaction (standards)
• Reference Standards
• Spheroids, Cellular
• Uterine Cervical Neoplasms (genetics, metabolism, pathology)