| Abstract | Immune sera from 11 patients cured of amebic liver abscess was used to identify antigens of Entamoeba histolytica. Strain HM1-IMSS, among the most virulent in axenic culture, was used. The 37 and 90 Kd antigens were surface glycoproteins as indicated by lactoperoxidase iodination and by Concanavalin A blotting; the 59 Kd antigen was a mannose containing glycoprotein that did not appear to be on the cell surface. Western blots of 11 different immune sera revealed specific binding of immune IgG to 9 amebic proteins. Most frequently recognized proteins were of molecular weight 37, 59, and 90 Kd. The immunoblot pattern in 5 patients was unchanged for up to 30 months post-treatment for liver abscess. |
| Authors | M P Joyce, J I Ravdin
(Affiliation: Department of Internal Medicine, University of Virginia School of Medicine, Charlottesville.)
|
| Journal | The American journal of tropical medicine and hygiene
(Am J Trop Med Hyg)
Vol. 38
Issue 1
Pg. 74-80
(Jan 1988)
ISSN: 0002-9637 UNITED STATES |
| PMID | 2893553
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
|
| Chemical References |
- Antibodies, Protozoan
- Antigens, Protozoan
- Antigens, Surface
- Immune Sera
|
| Topics |
- Animals
- Antibodies, Protozoan
(immunology)
- Antigens, Protozoan
(analysis, immunology)
- Antigens, Surface
(immunology)
- Electrophoresis, Polyacrylamide Gel
- Entamoeba histolytica
(immunology)
- Humans
- Immune Sera
(immunology)
- Immunoassay
- Liver Abscess, Amebic
(parasitology)
|
