Effects of beta 1- and beta 1 + beta 2-antagonists on training-induced myocardial hypertrophy and enzyme adaptation.

Abstract

The effects of beta 1- and beta 1 + beta 2-antagonists on the myocardial adaptation to exercise training were investigated in male Sprague-Dawley rats randomly divided into trained (treadmill, 1 hr/day, 5 days/week for 10 weeks at 27 m/min, 15% grade) without drug (TC), sedentary without drug (SC), trained treated with atenolol (TA) (10 mg/kg body wt, i.p.), trained treated with propranolol (TP, 30 mg/kg body wt, i.p.), and sedentary propranolol. Doses of both beta-antagonists were titrated to decrease the exercise heart rate by 25% compared to the controls. The heart weight and heart/body weight ratio were significantly greater in TC (1.28 +/- 0.07 g (P less than 0.01); 296 +/- 12 mg/100 g body wt (P less than 0.05) respectively) than in SC (1.09 +/- 0.04 g and 268 +/- 11 mg/100 g body wt), or in TP and TA. Myocardial mitochondrial protein was unchanged by training or beta-blockade. Citrate synthase and beta-hydroxyacyl CoA dehydrogenase activities were not altered. Carnitine palmitoyltransferase activity was increased in SP compared to SC. Training increased hexokinase activity only in TC (5.22 +/- 0.12 vs 4.26 +/- 0.23 mumol/min/g wet wt, P less than 0.01). Lactate dehydrogenase activity increased significantly (P less than 0.01) in both TC (383 +/- 14 mumol/min/g wet wt) and TA (372 +/- 14 mumol/min/g wet wt) compared to SC (276 +/- 14 mumol/min/g wet wt), but not in TP versus SP. These data indicate that (1) beta-adrenergic blockade prevents training-induced cardiac hypertrophy; (2) beta-antagonists have little effect on the myocardial oxidative capacity; and (3) while the training induction of myocardial hexokinase is inhibited by both beta 1- and beta 1 + beta 2-antagonists, myocardium may increase its ability to utilize lactate during exercise with training despite beta 1-blockade.

• Authors: L L Ji, F W Stratman, H A Lardy
• Journal: Biochemical pharmacology (Biochem Pharmacol) Vol. 36 Issue 20 Pg. 3411-7 (Oct 15 1987) ISSN: 0006-2952 [Print] England
• PMID: 2890350 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Adrenergic beta-Antagonists
• Lactates
• Proteins
• Lactic Acid
• 3-Hydroxyacyl CoA Dehydrogenases
• L-Lactate Dehydrogenase
• Carnitine O-Palmitoyltransferase
• Citrate (si)-Synthase
• Hexokinase

Topics

• 3-Hydroxyacyl CoA Dehydrogenases (analysis)
• Adaptation, Physiological (drug effects)
• Adrenergic beta-Antagonists (pharmacology)
• Animals
• Body Weight (drug effects)
• Cardiomegaly (prevention & control)
• Carnitine O-Palmitoyltransferase (analysis)
• Citrate (si)-Synthase (analysis)
• Heart Rate (drug effects)
• Hexokinase (analysis)
• L-Lactate Dehydrogenase (analysis)
• Lactates (metabolism)
• Lactic Acid
• Male
• Myocardium (enzymology)
• Oxidation-Reduction
• Physical Education and Training
• Proteins (analysis)
• Rats
• Rats, Inbred Strains