Recognition of the galactose- or N-acetylgalactosamine-binding lectin of Entamoeba histolytica by human immune sera.

Abstract

Cure of amebic liver abscess is associated with resistance to recurrent invasive amebiasis and the development of a humoral and cell-mediated immune response. We determined whether human immune sera contain blocking antibody for the 170-kilodalton (kDa) galactose or N-acetylgalactosamine (Gal/GalNAc)-binding lectin of Entamoeba histolytica. By Western blot (immunoblot) of whole amebae subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis, all eight immune sera studied here prominently recognized a 170-kDa amebic protein. Western blot of the purified Gal/GalNAc lectin with pooled human immune sera (PHIS) confirmed that the 170-kDa band was the adherence lectin. Immunoprecipitation of [35S]methionine-metabolically-labeled amebae with the antilectin monoclonal antibody H8-5 and with PHIS demonstrated that the 170-kDa lectin was the major antigen recognized by PHIS. The in vitro adherence of E. histolytica trophozoites to CHO cells at 4 degrees C was inhibited by prior exposure of amebae to greater than or equal to 1.0% PHIS. The humoral response to the Gal/GalNAc-binding lectin of the parasite may contribute to the development of protective immunity against invasive amebiasis.

• Authors: W A Petri Jr, M P Joyce, J Broman, R D Smith, C F Murphy, J I Ravdin
• Journal: Infection and immunity (Infect Immun) Vol. 55 Issue 10 Pg. 2327-31 (Oct 1987) ISSN: 0019-9567 [Print] United States
• PMID: 2888730 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies, Monoclonal
• Antibodies, Protozoan
• Antigens, Protozoan
• Antigens, Tumor-Associated, Carbohydrate
• Disaccharides
• Immune Sera
• Lectins
• Thomsen-Friedenreich antigen

Topics

• Adhesiveness
• Animals
• Antibodies, Monoclonal
• Antibodies, Protozoan (analysis)
• Antigens, Protozoan (immunology)
• Antigens, Tumor-Associated, Carbohydrate
• Autoradiography
• Binding, Competitive
• Chromatography, Affinity
• Disaccharides (immunology)
• Electrophoresis, Polyacrylamide Gel
• Entamoeba histolytica (immunology)
• Humans
• Immune Sera (analysis, immunology)
• Immunoassay
• Lectins (immunology)
• Liver Abscess, Amebic (immunology)