To prepare
ginsenoside-Rh₂
lipid nanoparticles, and investigate the synergistic effect with
borneol in resisting
tumor activity in vitro.
Ginsenoside-Rh₂
lipid nanoparticles were prepared by ultrasonic-assisted
solvent evaporation method, and orthogonal design was adopted to optimize formulation process. Its encapsulation efficiency,
drug loading ratio, particle size distribution, Zeta potential, morphology and in vitro drug release behavior were characterized, and synergistic effect with
borneol in resisting
tumor activity were preliminarily studied by MTT. These nanoemulsion particles prepared by the optimized process method were rounding and even in a good shape. Encapsulation efficiency and
drug loading ratio of three batches of nanoemulsion particles were (77.3±2.5)% and (7.2±0.2)%, respectively. Nanoemulsion particles showed an obvious sustained release characteristics, with 52.42% cumulative release within 96 h. The killing effect of nanoemulsion particles on
glioma cells was dose-dependent, with IC₅₀ of 22.33 μmol•L⁻¹ and 11.46 μmol•L⁻¹ after 24 h and 48 h, respectively. After the combination with
borneol, the killing effect of nanoemulsion particles on
glioma cells was dose-dependent, with IC₅₀ of 16.36 μmol•L⁻¹ and 8.04 μmol•L⁻¹ after 24 h and 48 h, respectively.