Efficiency of combined blocking of aerobic and glycolytic metabolism pathways in treatment of N1-S1 hepatocellular carcinoma in a rat model.

Abstract

BACKGROUND/AIM:

The aim of this study was to determine whether the addition of bumetanide (BU), a glycolytic metabolism pathway inhibitor, to arterial embolization improves tumor necrosis of N1-S1 hepatocellular carcinoma in a rat model.

MATERIALS AND METHODS:

N1-S1 tumors were surgically implanted in the liver of 14 Sprague-Dawley rats. The rats were divided into three groups: In control group (n = 5), 1 ml of normal saline was injected intra-arterially. The tumor in the transarterial embolization group (TAE, n = 4) was embolized using 10 mg of 50-150 μ polyvinyl alcohol (PVA) particles and embolization plus BU group (TAE + BU, n = 5) were embolized with 10 mg of PVA plus 0.04 mg/kg of BU. Tumor volume was measured using two-dimensional ultrasound before intervention and twice a week afterward. Relative tumor volume after the intervention was calculated as the percentage of preinterventional tumor volume. After 4 weeks of observation, the rats were sacrificed for histopathological evaluation.

RESULTS:

No statistically significant difference was detected in the preintervention tumor sizes between the three groups (P > 0.05). In the control group, the relative tumor volume increased to 142.5% larger than baseline measurements. In the TAE group, the tumor volume decreased by 18.2 ± 12.2%. The tumor volume in the TAE + BU group decrease by 90.4 ± 10.2%, which was 72.2% more than in TAE only group (P < 0.0001). Histopathological evaluation demonstrated no residual tumor in the TAE + BU group.

CONCLUSION:

Tumor necrosis significantly increased in N1-S1 tumor that received BU at the time of TAE when compared to TAE alone.

• Authors: Hooman Yarmohammadi, Luke R Wilkins, Joseph P Erinjeri, Ronald D Novak, Agata A Exner, Hanping Wu, Elena N Petre, Edward Boas, Etay Ziv, John R Haaga
• Journal: Journal of cancer research and therapeutics (J Cancer Res Ther) 2017 Jul-Sep Vol. 13 Issue 3 Pg. 533-537 ISSN: 1998-4138 [Electronic] India
• PMID: 28862222 (Publication Type: Journal Article)

Chemical References

• Bumetanide
• Polyvinyl Alcohol

Topics

• Aerobiosis (drug effects)
• Animals
• Antineoplastic Combined Chemotherapy Protocols (administration & dosage)
• Bumetanide (administration & dosage)
• Carcinoma, Hepatocellular (drug therapy, metabolism, pathology)
• Chemoembolization, Therapeutic
• Disease Models, Animal
• Glycolysis (drug effects)
• Humans
• Liver (drug effects, metabolism, pathology)
• Liver Neoplasms (drug therapy, metabolism, pathology)
• Polyvinyl Alcohol (administration & dosage)
• Rats
• Rats, Sprague-Dawley
• Tumor Burden (drug effects)