Inhibition of TRPM7 suppresses cell proliferation of colon adenocarcinoma in vitro and induces hypomagnesemia in vivo without affecting azoxymethane-induced early colon cancer in mice.

Abstract

BACKGROUND:

Magnesium (Mg2+) is an essential cation implicated in carcinogenesis, solid tumor progression and metastatic potential. The Transient Receptor Potential Melastatin Member 7 (TRPM7) is a divalent ion channel involved in cellular and systemic Mg2+ homeostasis. Abnormal expression of TRPM7 is found in numerous cancers, including colon, implicating TRPM7 in this process.

METHODS:

To establish a possible link between systemic magnesium (Mg2+) status, the Mg2+ conducting channel TRPM7 in colon epithelial cells, and colon carcinogenesis, in vitro whole-cell patch clamp electrophysiology, qPCR, and pharmacological tools were used probing human colorectal adenocarcinoma HT-29 as well as normal primary mouse colon epithelial cells. This was extended to and combined with aberrant crypt foci development in an azoxymethane-induced colorectal cancer mouse model under hypomagnesemia induced by diet or pharmacologic intervention.

RESULTS:

We find that TRPM7 drives colon cancer cell proliferation in human HT-29 and expresses in normal primary mouse colon epithelia. This is linked to TRPM7's dominant role as Mg2+ transporter, since high extracellular Mg2+ supplementation cannot rescue inhibition of cell proliferation caused by suppressing TRPM7 either genetically or pharmacologically. In vivo experiments in mice provide evidence that the specific TRPM7 inhibitor waixenicin A, given as a single bolus injection, induces transient hypomagnesemia and increases intestinal absorption of calcium. Repeated injections of waixenicin A over 3 weeks cause hypomagnesemia via insufficient Mg2+ absorption by the colon. However, neither waixenicin A, nor a diet low in Mg2+, affect aberrant crypt foci development in an azoxymethane-induced colorectal cancer mouse model.

CONCLUSION:

Early stage colon cancer proceeds independent of systemic Mg2+ status and TRPM7, and waixenicin A is a useful pharmacological tool to study of TRPM7 in vitro and in vivo.

• Authors: Junhao Huang, Hideki Furuya, Malika Faouzi, Zheng Zhang, Mahealani Monteilh-Zoller, Kelly Galbraith Kawabata, F David Horgen, Toshihiko Kawamori, Reinhold Penner, Andrea Fleig
• Journal: Cell communication and signaling : CCS (Cell Commun Signal) Vol. 15 Issue 1 Pg. 30 (08 15 2017) ISSN: 1478-811X [Electronic] England
• PMID: 28810912 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Acetates
• Diterpenes
• TRPM Cation Channels
• waixenicin A
• Trpm7 protein, mouse
• Azoxymethane
• Calcium

Topics

• Acetates (pharmacology)
• Adenocarcinoma (etiology, metabolism)
• Animals
• Azoxymethane (toxicity)
• Calcium (metabolism)
• Cell Proliferation (drug effects)
• Cells, Cultured
• Colonic Neoplasms (etiology, metabolism)
• Diterpenes (pharmacology)
• HT29 Cells
• Humans
• Intestinal Absorption
• Magnesium Deficiency (blood, etiology, metabolism)
• Male
• Mice
• Mice, Inbred C57BL
• TRPM Cation Channels (antagonists & inhibitors)