Plasma somatostatin-like immunoreactivity responses to a mixed meal and the heterogeneity in healthy and non-insulin-dependent (NIDDM) diabetics.

Abstract

Peripheral plasma somatostatin-like immunoreactivity (SLI) was estimated in non-extracted plasma using a specific somatostatin-14 (SS-14) antiserum. The basal plasma SLI level in healthy subjects (n = 18) was 43 +/- 2.9 pg/ml (mean +/- SE) and rose significantly to 8.3 +/- 2.7, 7.3 +/- 1.1 and 5.8 +/- 2.1 pg/ml above the mean basal level 20, 30, and 40 min after a mixed meal, respectively (P less than 0.05). Basal plasma SLI levels in diet (n = 8), sulfonyl urea (n = 8), and insulin groups (n = 8) of non-insulin-dependent maturity onset diabetics (NIDDM) were 50 +/- 1.6, 59 +/- 4.5, and 74 +/- 5.8 pg/ml, respectively. The basal levels for patients with NIDDM were significantly higher than those for healthy subjects (P less than 0.05). No significant increases in plasma SLI were observed after a mixed meal in any group of NIDDM subjects. Elevated plasma SLI levels are considered to be closely related to the severity of the diabetes. The ratios of SS-14 and SS-28 to the total amount of basal plasma SLI were analyzed using high pressure liquid chromatography (HPLC). The ratio of SS-14 to the total SLI was 71-80% in healthy subjects. The ratio of SS-28 to the total SLI increased from 26-30% in the diet group to 50-55% in the group on insulin. These findings suggest a possible pathophysiological role for gastrointestinal somatostatin in NIDDM.

• Authors: K Miyazaki, A Funakoshi, H Ibayashi
• Journal: Endocrinologia japonica (Endocrinol Jpn) Vol. 33 Issue 1 Pg. 51-9 (Feb 1986) ISSN: 0013-7219 [Print] Japan
• PMID: 2873028 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Blood Glucose
• Hypoglycemic Agents
• Immune Sera
• Insulin
• Somatostatin

Topics

• Adult
• Blood Glucose (analysis)
• Chromatography, High Pressure Liquid
• Cross Reactions
• Diabetes Mellitus, Type 2 (blood, drug therapy)
• Diet, Diabetic
• Eating
• Female
• Humans
• Hypoglycemic Agents (therapeutic use)
• Immune Sera
• Insulin (therapeutic use)
• Male
• Middle Aged
• Radioimmunoassay
• Reference Values
• Somatostatin (blood)