HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

An electrochemical sensor for indole in plasma based on MWCNTs-chitosan modified screen-printed carbon electrode.

Abstract
Indole is an essential metabolite in intestinal tract. The dysregulation of plasma indole concentration occurred in various diseases. In this study, the indole in plasma was determined directly using electrochemical sensor with multiwall carbon nanotubes-chitosan (MWCNTs-CS) modified screen-printed carbon electrode (SPCE). The electrochemical behavior of indole was elucidated by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) on the MWCNTs-CS composites modified SPCE (MWCNTs-CS/SPCE). The results showed that the current responses of indole improved greatly due to the high catalytic activity and electron transfer reaction of nano-composites. Under the optimized conditions, the linear range of indole was from 5 to 100μgL-1 with the detection limit of 0.5μgL-1 (S/N = 3). This novel electrochemical sensor exhibited acceptable accuracies and precisions with the variations less than 7.3% and 9.0%, respectively. Furthermore, high performance liquid chromatography (HPLC) method was utilized to compare with the established electrochemical method for the determination of indole in plasma. The results showed a high correlation between the two methods. At last, the electrochemical sensor was successfully applied to detect the level of indole in plasma samples with satisfactory selectivity and sensitivity. The concentrations of plasma indole in healthy pregnant women and gestational diabetes mellitus (GDM) patients were 5.3 (4.1-7.0)μgL-1 and 7.2 (4.5-9.4)μgL-1, respectively.
AuthorsMingchao Jin, Xiaoqing Zhang, Qianna Zhen, Yifan He, Xiao Chen, Wenjing Lyu, Runchuan Han, Min Ding
JournalBiosensors & bioelectronics (Biosens Bioelectron) Vol. 98 Pg. 392-397 (Dec 15 2017) ISSN: 1873-4235 [Electronic] England
PMID28709089 (Publication Type: Journal Article)
CopyrightCopyright © 2017 Elsevier B.V. All rights reserved.
Chemical References
  • Indoles
  • Nanotubes, Carbon
  • indole
  • Chitosan
Topics
  • Biosensing Techniques (methods)
  • Chitosan (chemistry)
  • Electrochemical Techniques (methods)
  • Electrodes
  • Female
  • Humans
  • Indoles (blood)
  • Limit of Detection
  • Nanotubes, Carbon (chemistry)
  • Pregnancy

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: