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Biopolymer zein-coated gold nanoparticles: Synthesis, antibacterial potential, toxicity and histopathological effects against the Zika virus vector Aedes aegypti.

Abstract
The control of multidrug-resistant bacteria as well as insect pests and vectors is timely and important now a days. The present study was designed to evaluate the in vitro antibacterial, antibiofilm and mosquito larvicidal effects of gold nanoparticles synthesized using the zein biopolymer (Ze-AuNPs) against Gram positive (Bacillus pumilus and Bacillus subtilis), Gram negative (Shigella sonnei and Pseudomonas aeruginosa) bacteria and third instar larvae of the dengue and Zika virus vector Aedes aegypti. The synthesized Ze-AuNPs were characterized by UV-vis spectroscopy, X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The antibacterial assays testing Ze-AuNPs at 100μg/ml showed that the zones of inhibition against Gram positive species B. pumilus and B. subtilis were 13.9 and 14.2mm, respectively, while for Gram negative S. sonnei and P. aeruginosa they were 18.1 and 18.4mm, respectively. Light and confocal laser scanning microscopy (CLSM) confirmed the interruption and disintegration of bacterial biofilm post-treatment with Ze-AuNPs at 100μg/ml. In larvicidal assays on A. aegypti, HAuCl4 and Ze-AuNPs treated third instar larvae of A. aegypti showed LC50 of 26.6 and 6.81mg/L, respectively, and LC90 of 81.1 and 13.6mg/L respectively. The histopathological analysis of A. aegypti treated with Ze-AuNPs showed complete disintegration of abdominal region, particularly the midgut and caeca, with loss of lateral and caudal hairs. The stereomicroscopic visualization of A. aegypti treated with Ze-AuNPs showed the loss of upper head hair, lower head hair, antenna hair, lateral hair and caudal hair. Overall, the study concludes that Ze-AuNPs have excellent antibacterial, antibiofilm effects and has ability to control the larval populations of A. aegypti mosquitoes.
AuthorsPeriyakaruppan Suganya, Baskaralingam Vaseeharan, Sekar Vijayakumar, Banumathi Balan, Marimuthu Govindarajan, Naiyf S Alharbi, Shine Kadaikunnan, Jamal M Khaled, Giovanni Benelli
JournalJournal of photochemistry and photobiology. B, Biology (J Photochem Photobiol B) Vol. 173 Pg. 404-411 (Aug 2017) ISSN: 1873-2682 [Electronic] Switzerland
PMID28654862 (Publication Type: Journal Article)
CopyrightCopyright © 2017 Elsevier B.V. All rights reserved.
Chemical References
  • Anti-Bacterial Agents
  • Biopolymers
  • Drug Carriers
  • Insecticides
  • Gold
  • Zein
Topics
  • Aedes (growth & development, virology)
  • Animals
  • Anti-Bacterial Agents (chemical synthesis, chemistry, pharmacology)
  • Biofilms (drug effects)
  • Biopolymers (chemistry)
  • Drug Carriers (chemistry)
  • Gold (chemistry)
  • Gram-Negative Bacteria (drug effects, physiology)
  • Gram-Positive Bacteria (drug effects, physiology)
  • Insect Vectors (drug effects)
  • Insecticides (chemistry, toxicity)
  • Larva (drug effects, virology)
  • Metal Nanoparticles (chemistry)
  • Microbial Sensitivity Tests
  • Microscopy, Confocal
  • Particle Size
  • Zein (chemistry, pharmacology, toxicity)
  • Zika Virus (physiology)

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