Influence on cell-cell communication (dye-transfer) of the oncogenic beta-blocker DL-ZAMI 1305: possible relation to tumor promotion.

Abstract

The effect of the oncogenic beta-blocker DL-1-(2-nitro-3-methyl-phenoxy)-3-tert-butyl-amino-propan-2-ol (DL-ZAMI 1305) on intercellular communication between cultured cells was studied. Intercellular communication of Chinese hamster V79 cells was measured by the dye-transfer method in which the spread of intracellularly microinjected fluorescent probe, Lucifer Yellow CH, through gap-junctions was used as an index of intercellular communication. When V79 cells are cultured with non-toxic doses (1-60 micrograms/ml) of DL-ZAMI 1305, a significant inhibition of dye-transfer is observed after 4 h. The inhibition is dose-related and greater than 90% inhibition is seen at the dose of 50 micrograms/ml. When DL-ZAMI 1305 is added at 0 and 24 h of experiment, its inhibitory effect is maintained for at least 48 h at high doses (50-60 micrograms/ml), whereas for lower doses of DL-ZAMI 1305, some recovery is seen after 24 h incubation. These results are suggestive of a possible tumor-promoting activity of DL-ZAMI 1305; in vivo studies on this carcinogen are in progress.

• Authors: G Mazzoleni, G Ragnotti, T Enomoto, H Yamasaki
• Journal: Carcinogenesis (Carcinogenesis) Vol. 6 Issue 10 Pg. 1477-82 (Oct 1985) ISSN: 0143-3334 [Print] England
• PMID: 2864144 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Adrenergic beta-Antagonists
• Carcinogens
• Isoquinolines
• Propanolamines
• ZAMI 1305
• lucifer yellow

Topics

• Adrenergic beta-Antagonists (pharmacology)
• Animals
• Carcinogens (pharmacology)
• Cell Communication (drug effects)
• Cell Division (drug effects)
• Cells, Cultured
• Cricetinae
• Cricetulus
• Dose-Response Relationship, Drug
• Isoquinolines
• Propanolamines (pharmacology)
• Time Factors