Abstract |
Human pluripotent stem cells (hPSC) require signaling provided by fibroblast growth factor ( FGF) receptors. This can be initiated by the recombinant FGF2 ligand supplied exogenously, but hPSC further support their niche by secretion of endogenous FGF2. In this study, we describe a role of tyrosine kinase expressed in hepatocellular carcinoma ( TEC) kinase in this process. We show that TEC-mediated FGF2 secretion is essential for hPSC self-renewal, and its lack mediates specific differentiation. Following both short hairpin RNA- and small interfering RNA-mediated TEC knockdown, hPSC secretes less FGF2. This impairs hPSC proliferation that can be rescued by increasing amounts of recombinant FGF2. TEC downregulation further leads to a lower expression of the pluripotency markers, an improved priming towards neuroectodermal lineage, and a failure to develop cardiac mesoderm. Our data thus demonstrate that TEC is yet another regulator of FGF2-mediated hPSC pluripotency and differentiation. Stem Cells 2017;35:2050-2059.
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Authors | Tereza Vanova, Zaneta Konecna, Zuzana Zbonakova, Giuseppe La Venuta, Karolina Zoufalova, Sarka Jelinkova, Miroslav Varecha, Vladimir Rotrekl, Pavel Krejci, Walter Nickel, Petr Dvorak, Michaela Kunova Bosakova |
Journal | Stem cells (Dayton, Ohio)
(Stem Cells)
Vol. 35
Issue 9
Pg. 2050-2059
(09 2017)
ISSN: 1549-4918 [Electronic] England |
PMID | 28631381
(Publication Type: Journal Article)
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Copyright | © 2017 AlphaMed Press. |
Chemical References |
- Biomarkers
- Recombinant Proteins
- Fibroblast Growth Factor 2
- Tec protein-tyrosine kinase
- Protein-Tyrosine Kinases
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Topics |
- Biomarkers
(metabolism)
- Cell Line
- Cell Lineage
(drug effects)
- Cell Proliferation
(drug effects)
- Down-Regulation
(drug effects)
- Fibroblast Growth Factor 2
(metabolism)
- Humans
- Pluripotent Stem Cells
(cytology, enzymology)
- Protein-Tyrosine Kinases
(metabolism)
- Recombinant Proteins
(pharmacology)
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