Measurement of propericiazine in plasma by capillary gas chromatography and selected ion monitoring detection.

Abstract

A new method, based upon a selective extraction and gas chromatographic/mass spectrometric analysis, was developed to monitor the effect of combined haemoperfusion-haemodialysis treatment in a case of propericiazine poisoning. The method relies on the selected ion monitoring of the acetate derivatives of propericiazine and its internal standard fluphenazine, after their extraction from 1 ml of alkalinized plasma with n-hexane:isopropanol (8:2, v/v), back-extraction into an acidified water phase, realkalinization and extraction with n-hexane: isopropanol, derivatization with acetic anhydride and gas chromatography on a short (12 m) OV-101 fused silica capillary column. The described procedure is specific and provides between-assay variability of 4.8% CV at 5 micrograms 1(-1) plasma concentration. The method enables quantification down to 1 microgram 1(-1) and hence demonstrates sufficient sensitivity to permit pharmacokinetic or drug monitoring studies.

• Authors: A P De Leenheer, J A Jonckheere, M F Lefevere, M E De Broe, G A Verpooten
• Journal: Biomedical mass spectrometry (Biomed Mass Spectrom) Vol. 12 Issue 1 Pg. 25-9 (Jan 1985) ISSN: 0306-042X [Print] England
• PMID: 2859055 (Publication Type: Case Reports, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Phenothiazines

Topics

• Female
• Gas Chromatography-Mass Spectrometry (methods)
• Hemoperfusion
• Humans
• Middle Aged
• Phenothiazines (blood, poisoning)
• Renal Dialysis
• Suicide, Attempted