Detection of a 140 KDA nucleolar protein with an anti-PCNA autoantibody.

Abstract

To assess the numbers and types of PCNA (proliferating cell nuclear antigen) species, immunoprecipitation studies on HeLa cell, nuclear and nucleolar extracts were performed. A 140 KDa protein from HeLa nucleoli was immunoprecipitated by an autoantibody (E.B.) previously used to detect the proliferating cell nuclear antigens (PCNA). The 140 KDa protein was also detected in the nuclear extract of colon carcinoma cells (omega) labeled in vitro with 125I-Bolton Hunter reagent. When the growth of the colon carcinoma cells was inhibited by 1% N,N-dimethylformamide for two weeks, the 140 KDa protein was not detected which suggests this protein is associated with cell growth.

• Authors: P K Chan, M Aldrich, E M Tan, H Busch
• Journal: Cell biology international reports (Cell Biol Int Rep) Vol. 9 Issue 1 Pg. 61-8 (Jan 1985) ISSN: 0309-1651 [Print] England
• PMID: 2858268 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Autoantibodies
• Nuclear Proteins
• Nucleoproteins
• Proliferating Cell Nuclear Antigen
• Ribonucleoproteins
• Nucleophosmin

Topics

• Autoantibodies (immunology)
• Cell Division
• Cells, Cultured
• Colonic Neoplasms (immunology)
• HeLa Cells (immunology)
• Humans
• Molecular Weight
• Nuclear Proteins
• Nucleophosmin
• Nucleoproteins (immunology, isolation & purification)
• Proliferating Cell Nuclear Antigen
• Ribonucleoproteins (immunology)