Differential resistance to growth of a tumor expressing incompatible minor alloantigens reflects regulatory influences rather than differences in anti-minor-CTL-P frequencies.

Abstract

In previous studies it was found that BALB/c (H-2d) was more susceptible than (BALB/c X A)F1 (H-2d X H-2a) to a tumor bearing multiple mismatched minor histocompatibility antigens, the DBA/2 (H-2d) mastocytoma P815, and that this resistance was H-2 linked. In the present studies the immunologic basis of this effect was examined by comparing the cytotoxic-T-lymphocyte (CTL) responses of BALB/c with those of (BALB/c X A)F1. Despite the BALB/c X A)F1's 34-fold greater resistance to P815 in vivo, the numbers of effector cell precursors were found to be similar in the two hosts as shown by (a) similar anti-P815 CTL responses in vitro with T-cell growth factor, (b) similar secondary anti-DBA/2 MiHA responses after in vivo priming with irradiated P815, and (c) similar frequencies of anti-DBA/2 CTL precursors by limiting-dilution analysis. However, priming with proliferating P815 in vivo revealed a defect in the BALB/c animals: Spleen cells from such animals were unable to control the growth of contaminating P815 cells in vitro or to mount strong secondary CTL responses to DBA/2 antigens. The defective priming of BALB/c could be corrected when DBA/2 spleen cells were added to the P815 inoculum. This impaired priming by living tumor cells was not seen in (BALB/c X A)F1. It is concluded that the use of living P815 tumor cells revealed a defect in immunoregulation in BALB/c mice, which rendered them susceptible to tumor growth in spite of apparently adequate numbers of anti-minor-CTL precursors. How the additional H-2 products expressed in the (BALB/c X A)F1 might correct this defect is discussed.

• Authors: G Carayanniotis, P F Halloran
• Journal: Cellular immunology (Cell Immunol) Vol. 91 Issue 1 Pg. 100-10 (Mar 1985) ISSN: 0008-8749 [Print] Netherlands
• PMID: 2857596 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Neoplasm
• Interleukin-2

Topics

• Animals
• Antigens, Neoplasm (administration & dosage, immunology)
• Cytotoxicity Tests, Immunologic
• Female
• Immunity, Innate
• Immunization, Secondary
• Interleukin-2 (physiology)
• Lymphocyte Transfusion
• Lymphocytes (radiation effects)
• Lymphoma (genetics, immunology)
• Mast-Cell Sarcoma (genetics, immunology)
• Mice
• Mice, Inbred BALB C
• Mice, Inbred C57BL
• Mice, Inbred DBA
• Minor Histocompatibility Loci
• Neoplasm Transplantation
• Rats
• Rats, Inbred Lew
• Stem Cells (immunology)
• T-Lymphocytes (immunology)
• T-Lymphocytes, Cytotoxic (immunology)