18-Des-hydroxy Cytochalasin: an antiparasitic compound of Diaporthe phaseolorum-92C, an endophytic fungus isolated from Combretum lanceolatum Pohl ex Eichler.

Abstract

Chemical investigation of the ethyl acetate extract from the endophytic fungus Diaporthe phaseolorum-92C (92C) isolated from the roots of Combretum lanceolatum led to the isolation of 18-des-hydroxy Cytochalasin H (compound 1). The trypanocidal and schistosomicidal activity and cytotoxicity of the extract from 92C were evaluated. The schistosomicidal, leishmanicidal, antimicrobial, and antioxidant actions, as well as the antitumor activity against the breast cancer cells MDA-MB-231 and MCF-7, and the cytotoxicity towards normal human lung fibroblasts GM07492A of compound 1 was tested. The extract from 92C (20 μg/mL) exerted potent trypanocidal activity, reducing 82% of the number of amastigotes and trypomastigotes of Trypanosoma cruzi. Compound 1 at 50 μg/mL killed 50% of Schistosoma mansoni adult worms. Compound 1 reduced the viability of Leishmania amazonenses promastigotes (IC50 = 9.2 μg/mL) and of the cancer cells MDA-MB-231 and MCF-7 (IC50 = 17.5 and 8.88 μg/mL, respectively), presented moderate antioxidant activity, and gave IC50 of 2049.7 ± 39.9 μg/mL for the cytotoxicity towards normal cells GM07492A. This knowledge is highly relevant to the search for new promising compounds for therapeutic purposes.

• Authors: Elson Rudimar Brissow, Igor Pereira da Silva, Kátia Aparecida de Siqueira, Jaqueline Alves Senabio, Leticia Pereira Pimenta, Ana Helena Januário, Lizandra Guidi Magalhães, Ricardo Andrade Furtado, Denise Crispim Tavares, Policarpo Ademar Sales Junior, Jane Lima Santos, Marcos Antônio Soares
• Journal: Parasitology research (Parasitol Res) Vol. 116 Issue 7 Pg. 1823-1830 (Jul 2017) ISSN: 1432-1955 [Electronic] Germany
• PMID: 28497228 (Publication Type: Journal Article)

Chemical References

• Antiparasitic Agents
• Cytochalasins
• Schistosomicides
• Trypanocidal Agents
• cytochalasin H

Topics

• Animals
• Antiparasitic Agents (isolation & purification, pharmacology)
• Ascomycota (chemistry)
• Combretum (microbiology)
• Cytochalasins (isolation & purification, pharmacology)
• Endophytes
• Female
• Humans
• Leishmania (drug effects)
• Male
• Mice, Inbred BALB C
• Schistosomicides (pharmacology)
• Trypanocidal Agents (pharmacology)
• Trypanosoma cruzi (drug effects)