Analysis of simian virus 40 infection of CV-1 cells by quantitative two-color fluorescence with flow cytometry.

Abstract

Quantitative two-color fluorescent analysis of Simian virus (SV40) infection of permissive CV-1 cells was investigated. Analysis included by quantitation of cellular DNA, the early viral tumor (T) antigen with a monoclonal antibody, and late viral (V) antigens with a polyclonal antibody. T antigen was detected in all phases of the cell cycle at 6 and 12 h, after SV40 infection of growth arrested cells. At later time intervals, the percentage of T-antigen-positive cells increased with the induction of the cells into successive rounds of DNA synthesis and an increase in tetraploid-polyploid cells. The amount of T antigen per cell increased as the cells entered the successive stages of the cell cycle (G0/G1----G2 + M----tetraploid S and G2 + M). The V antigen from adsorbed virus was detected immediately after infection. Synthesis of V antigen began in late S and G2 + M phases of the cell cycle. This quantitative analysis allows a definitive determination of antigen per cell in a population correlated with the cell cycle and may be useful in correlating viral and cellular events with transformation.

• Authors: J M Lehman, J Laffin, J W Jacobberger, D Fogleman
• Journal: Cytometry (Cytometry) Vol. 9 Issue 1 Pg. 52-9 (Jan 1988) ISSN: 0196-4763 [Print] United States
• PMID: 2842119 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antigens, Viral, Tumor
• DNA, Viral

Topics

• Animals
• Antigens, Viral, Tumor (biosynthesis)
• Cell Cycle
• Cell Line
• Chlorocebus aethiops
• DNA, Viral (analysis)
• Flow Cytometry (methods)
• Fluorescent Antibody Technique
• Kidney (cytology, microbiology)
• Methods
• Simian virus 40
• Tumor Virus Infections (metabolism)