Abstract |
Inhibition of intercellular communication has been hypothesized to play a role in tumor promotion. The compound 2,2',4,4',5,5'-hexabromobiphenyl (245-HBB) is a tumor promoter in vivo and blocks intercellular communication in vitro. The scrape-loading/dye-transfer (SL/DT) assay was used to assess this in vitro effect at varying concentrations of 245-HBB. The SL/DT technique is based on the intracellular loading of a fluorescent dye, lucifer yellow (LY), and monitoring its transfer into adjacent cells via patent gap junctions. Confluent WB-F344 (rat epithelial) cells were exposed to various noncytolethal concentrations of 245-HBB. Transfer of LY was then quantified with anchored cell analysis/sorting (ACAS 470, Meridian Instruments, Okemos, Mich.). The results indicate an inverse correlation between the degree of fluorescence in secondary LY-recipient cells and the treatment concentration. The coupling of these two new methods of cellular biology provided rapid quantitative analysis of dye transfer in measuring the concentration/response of modulation of gap-junctional permeability in cultured cells.
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Authors | M G Evans, M H el-Fouly, J E Trosko, S D Sleight |
Journal | Journal of toxicology and environmental health
(J Toxicol Environ Health)
Vol. 24
Issue 2
Pg. 261-71
( 1988)
ISSN: 0098-4108 [Print] United States |
PMID | 2838643
(Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Carcinogens
- Isoquinolines
- Polybrominated Biphenyls
- lucifer yellow
- 2,4,5,2',4',5'-hexabromobiphenyl
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Topics |
- Animals
- Carcinogens
- Cell Communication
(drug effects)
- In Vitro Techniques
- Intercellular Junctions
(drug effects)
- Isoquinolines
- Male
- Microscopy, Fluorescence
- Polybrominated Biphenyls
(toxicity)
- Rats
- Rats, Inbred F344
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