Photoaffinity labeling of a 33 kDa protein subunit of the delta-opioid receptor in neuroblastoma and hybrid cell lines.

Abstract

Tritiated DTLET (Tyr-D-Thr-Gly-Phe-Leu-Thr) binds with high affinity, specificity and saturability to neuroblastoma N18TG2 and hybrid neuroblastoma x glioma NG108-15 and NG108-5 intact cells. The delta-opioid receptor density in cells cultured in chemically defined medium was increased about 2 times compared to that in cells cultured in 10% fetal calf serum. A major and a minor protein species covalently and specifically bound to [125I]azido-DTLET (Tyr-D-Thr-Gly-pN3Phe-Leu-Thr), photoactivatable ligand, migrated on SDS-gel electrophoresis with Mr values near 33,000 and 58,000, respectively.

• Authors: C Icard-Liepkalns, P Bochet
• Journal: FEBS letters (FEBS Lett) Vol. 233 Issue 1 Pg. 167-72 (Jun 06 1988) ISSN: 0014-5793 [Print] England
• PMID: 2838323 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Affinity Labels
• Azides
• Iodine Radioisotopes
• Oligopeptides
• Receptors, Opioid
• Receptors, Opioid, delta
• Tritium
• deltakephalin
• tyrosyl-threonyl-glycyl-(4-azidophenylalanyl)-leucyl-threonine

Topics

• Affinity Labels
• Animals
• Azides (metabolism)
• Electrophoresis, Polyacrylamide Gel
• Glioma (metabolism)
• Hybrid Cells (metabolism)
• Iodine Radioisotopes
• Kinetics
• Mice
• Neuroblastoma (metabolism)
• Oligopeptides (metabolism)
• Photochemistry
• Rats
• Receptors, Opioid (metabolism)
• Receptors, Opioid, delta
• Tritium
• Tumor Cells, Cultured