Truncated glucagon-like peptide-1 (proglucagon 78-107 amide), an intestinal insulin-releasing peptide, has specific receptors on rat insulinoma cells (RIN 5AH).

Abstract

We studied binding of 125I-labelled truncated-glucagon-like peptide-1 (proglucagon 78-107 amide) to a cloned rat insulin-producing cell line, RIN 5AH, in monolayer culture. Interaction of the peptide with pancreatic insulinoma cells was saturable and time dependent. Half-maximal binding was obtained when the cells were incubated in the presence of 3.3 x 10(-9) mol/l unlabelled truncated-glucagon-like peptide-1 (proglucagon 78-107 amide). Neither glucagon, full-length glucagon-like peptide-1 (proglucagon 72-107 amide) nor gastric inhibitory peptide competed for binding in concentrations up to 10(-6) mol/l.

• Authors: C Orskov, J H Nielsen
• Journal: FEBS letters (FEBS Lett) Vol. 229 Issue 1 Pg. 175-8 (Feb 29 1988) ISSN: 0014-5793 [Print] England
• PMID: 2831085 (Publication Type: Journal Article)

Chemical References

• Glp1r protein, rat
• Glucagon-Like Peptide-1 Receptor
• Insulin
• Peptides
• Receptors, Cell Surface
• Receptors, Glucagon
• Glucagon-Like Peptide 1
• Glucagon

Topics

• Adenoma, Islet Cell (analysis)
• Animals
• Binding, Competitive
• Glucagon (metabolism)
• Glucagon-Like Peptide 1
• Glucagon-Like Peptide-1 Receptor
• Insulin (metabolism)
• Insulinoma (analysis)
• Intestinal Mucosa (metabolism)
• Intestines (drug effects)
• Kinetics
• Pancreatic Neoplasms (analysis)
• Peptides (metabolism)
• Rats
• Receptors, Cell Surface (metabolism)
• Receptors, Glucagon