Inhibition of LTB4 binding to human neutrophils by nordihydroguaiaretic acid.

Abstract

Nordihydroguaiaretic acid (NDGA) was investigated for its ability to interact with leukotriene B4 receptors on human polymorphonuclear leukocytes (hPMNs). 3H-LTB4 binding to specific receptors was reduced in a dose-dependent manner with maximal reduction at 100 microM NDGA and an IC50 of about 50 microM. Binding of another inflammatory stimulus. N-formyl-norleucyl-leucyl-phenylalanine (FNLP) was not affected by similar treatment. Chemotaxis and enzyme release stimulated by LTB4 and oligopeptide were inhibited by NDGA. In addition, LTB4-triggered inflammation in vivo in mice was inhibited by systemic administration of NDGA. These data suggest that LTB4 receptor antagonism may contribute to inhibition of inflammation by NDGA.

• Authors: B L Maloff, D Fefer, G M Cooke, N R Ackerman
• Journal: Agents and actions (Agents Actions) Vol. 21 Issue 3-4 Pg. 358-60 (Aug 1987) ISSN: 0065-4299 [Print] Switzerland
• PMID: 2825484 (Publication Type: Journal Article)

Chemical References

• Catechols
• Receptors, Immunologic
• Receptors, Leukotriene B4
• Leukotriene B4
• Masoprocol

Topics

• Animals
• Catechols (pharmacology)
• Humans
• In Vitro Techniques
• Inflammation (chemically induced, prevention & control)
• Leukotriene B4 (metabolism, pharmacology)
• Masoprocol (pharmacology)
• Mice
• Neutrophils (drug effects, metabolism)
• Receptors, Immunologic (drug effects)
• Receptors, Leukotriene B4