Abstract |
We examined the effects of agents/conditions that raise intracellular cAMP concentration in the induction of the 47,000-dalton RI cAMP- binding protein in the "neurite-minus" N1A-103 mouse neuroblastoma cells. The amount of RI in cell extracts was quantitated by photoaffinity labeling with 8-N3-[32P]cAMP and by ELISA and Western blot. Our results showed that treatment of the N1A-103 neuroblastoma cells with 20 microM forskolin or 1 mM dibutyryl cAMP, or reducing the serum concentration in the culture medium from 10% to 1% caused a 3-4 fold increase in the amount of RJ. Our results suggest that the induction of RJ can occur independent of morphological differentiation in the mouse neuroblastoma cells.
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Authors | A Y Liu, V Kamalakannan, Z Lin |
Journal | Biochemical and biophysical research communications
(Biochem Biophys Res Commun)
Vol. 148
Issue 1
Pg. 350-6
(Oct 14 1987)
ISSN: 0006-291X [Print] United States |
PMID | 2823808
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Affinity Labels
- Azides
- Carrier Proteins
- Cyclic AMP Receptor Protein
- 8-azidoadenosine-3',5'-monophosphate
- Cyclic AMP
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Topics |
- Affinity Labels
(metabolism)
- Animals
- Azides
(metabolism)
- Carrier Proteins
(analysis, biosynthesis, metabolism)
- Cell Line
- Cyclic AMP
(analogs & derivatives, metabolism)
- Cyclic AMP Receptor Protein
- Cytosol
(metabolism)
- Enzyme-Linked Immunosorbent Assay
- Kinetics
- Mice
- Neuroblastoma
(metabolism)
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