Proteins are cointernalized with virion particles during early infection.

Abstract

Mammalian cells infected with enveloped or naked animal viruses become permeabilized to several proteins. The entry of alpha-sarcin, horseradish peroxidase, and luciferase is greatly increased during the early stages of viral infection. This process is promoted by uv-inactivated SFV, but not by heat-inactivated virions, suggesting that the process does not require viral gene expression. The entry of alpha-sarcin has been monitored both by its effects on protein synthesis and by indirect immunofluorescence. Increased entry of alpha-sarcin and luciferase is clearly observed in animal virus-infected cells by fluorescence microscopy. Chloroquine blocks the coentry of alpha-sarcin with enveloped, but not with naked, viruses. These results have implications to elucidate the mechanisms involved in virus entry.

• Authors: M J Otero, L Carrasco
• Journal: Virology (Virology) Vol. 160 Issue 1 Pg. 75-80 (Sep 1987) ISSN: 0042-6822 [Print] United States
• PMID: 2820142 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Fungal Proteins
• Proteins
• alpha-sarcin
• Chloroquine
• Horseradish Peroxidase
• Luciferases
• Endoribonucleases

Topics

• Adenoviruses, Human
• Animals
• Cell Membrane Permeability
• Chloroquine (pharmacology)
• Endocytosis (drug effects)
• Endoribonucleases
• Fungal Proteins (metabolism, pharmacology)
• HeLa Cells (physiology)
• Horseradish Peroxidase (metabolism)
• Luciferases (metabolism)
• Poliovirus
• Protein Biosynthesis (drug effects)
• Proteins (metabolism)
• Semliki forest virus
• Vero Cells (physiology)
• Vesicular stomatitis Indiana virus
• Virion