Physapubescin, a natural withanolide as a kidney-type glutaminase (KGA) inhibitor.

Abstract

Kidney-type glutaminase (KGA) is over expressed in many kinds of cancers that converts glutamine to glutamate for supplying energy, and has become an object for targeted cancer therapy. The structure-based virtual ligand screening identified physapubescin, a withanolide purified from Physalis pubescens L., as a possible inhibitor of KGA with low binding energy. Enzyme inhibition experiments and cell-based assays further confirmed its inhibitory effects on KGA activity, suggesting potential applications of physapubescin and its derivatives as KGA inhibitors.

• Authors: Li Cheng, Can-Rong Wu, Li-Han Zhu, Hua Li, Li-Xia Chen
• Journal: Bioorganic & medicinal chemistry letters (Bioorg Med Chem Lett) Vol. 27 Issue 5 Pg. 1243-1246 (03 01 2017) ISSN: 1464-3405 [Electronic] England
• PMID: 28174105 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2017 Elsevier Ltd. All rights reserved.

Chemical References

• Antineoplastic Agents
• Ligands
• Withanolides
• physapubescin
• Glutaminase

Topics

• Animals
• Antineoplastic Agents (chemistry, pharmacology)
• Biological Assay
• Cell Line, Tumor
• Enzyme Activation (drug effects)
• Glutaminase (antagonists & inhibitors)
• Humans
• Inhibitory Concentration 50
• Kidney (enzymology)
• Ligands
• Molecular Docking Simulation
• Molecular Structure
• Solanaceae (chemistry)
• Withanolides (chemistry, pharmacology)