Helodermin-like peptides in noradrenaline cells of adrenal medulla.

Abstract

Helodermin, a VIP/secretin-like peptide, was first isolated from the venom of the lizard Gila monster. Small amounts of helodermin-like peptides have since been detected in many mammalian tissues. Notably high concentrations were demonstrated in the thyroid gland, and immunocytochemical studies revealed intense helodermin-like immunostaining in thyroid C cells and medullary thyroid carcinoma cells. In the present study, we examined the adrenal gland of mouse, rat and pig for the presence of helodermin-like peptides. Using an antiserum raised against lizard helodermin immunostaining was observed in the noradrenaline-producing cells of the adrenal medulla in all 3 species. Radioimmunoassay revealed high concentrations of helodermin-like peptides in the mouse and rat adrenal. The concentrations in the pig adrenal could not be determined because of a non-parallel dilution curve. Upon high-performance liquid chromatography, the immunoreactive material in extracts of mouse and rat adrenals eluted in one major peak, close to the elution position of lizard helodermin.

• Authors: A Bjartell, P Persson, A Absood, F Sundler, R Håkanson
• Journal: Regulatory peptides (Regul Pept) Vol. 26 Issue 1 Pg. 27-34 (Aug 1989) ISSN: 0167-0115 [Print] Netherlands
• PMID: 2813855 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Intercellular Signaling Peptides and Proteins
• Peptides
• heliodermin
• Norepinephrine

Topics

• Adrenal Medulla (chemistry, cytology)
• Animals
• Chromatography, High Pressure Liquid
• Immunochemistry
• Intercellular Signaling Peptides and Proteins
• Lizards
• Male
• Mice
• Norepinephrine (analysis)
• Peptides
• Rats
• Swine