Abstract | OBJECTIVE: METHODS: A total of 97 patients with pleural effusion specimens were enrolled from The First Affiliated Hospital of Zhengzhou University between June 2014 and December 2015. All cases were categorized into malignant pleural effusion group (n = 55) and benign pleural effusion group (n = 42) according to etiologies. Enzyme-linked immunosorbent assay was applied to examine the levels of sB7-H4 in pleural effusion and meanwhile CEA concentrations were detected by electro-chemiluminescence immunoassays. Receiver operating characteristic (ROC) curve was established to assess the diagnostic value of sB7-H4 and CEA in pleural effusion. The correlation between sB7-H4 and CEA levels was analyzed by Pearson's product-moment. RESULTS: The concentrations of sB7-H4 and CEA in MPE exhibited obviously higher than those of BPE ([60.08 ± 35.04] vs. [27.26 ± 9.55] ng/ml, P = .000; [41.49 ± 37.16] vs. [2.41 ± 0.94] ng/ml, P = .000). The AUC area under ROC curve of sB7-H4 and CEA was 0.884 and 0.954, respectively. Two cutoff values by ROC curve analysis of sB7-H4 36.5 ng/ml and CEA 4.18 ng/ml were obtained, with a corresponding sensitivity (81.82%, 87.28%), specificity (90.48%, 95.24%), accuracy (85.57%, 90.72%), positive predictive value (PPV) (91.84%, 96.0%), negative predictive value (NPV) (79.17%, 85.11%), positive likelihood ratio (PLR) (8.614, 18.327), and negative likelihood ratio (NLR) (0.201, 0.134). When sB7-H4 and CEA were combined to detect pleural effusion, it obtained a higher sensitivity 90.91% and specificity 97.62%. Furthermore, correlation analysis result showed that the level of sB7-H4 was correlated with CEA level (r = .770, P = .000). CONCLUSIONS: sB7-H4 was a potentially valuable tumor marker in the differentiation between BPE and MPE. The combined detection of sB7-H4 and CEA could improve the diagnostic sensitivity and specificity for MPE.
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Authors | Xiaogang Jing, Fei Wei, Jing Li, Lingling Dai, Xi Wang, Liuqun Jia, Huan Wang, Lin An, Yuanjian Yang, Guojun Zhang, Zhe Cheng |
Journal | The clinical respiratory journal
(Clin Respir J)
Vol. 12
Issue 3
Pg. 986-990
(Mar 2018)
ISSN: 1752-699X [Electronic] England |
PMID | 28127951
(Publication Type: Journal Article)
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Copyright | © 2017 John Wiley & Sons Ltd. |
Chemical References |
- Biomarkers, Tumor
- Carcinoembryonic Antigen
- V-Set Domain-Containing T-Cell Activation Inhibitor 1
- VTCN1 protein, human
|
Topics |
- Aged
- Biomarkers, Tumor
(metabolism)
- Carcinoembryonic Antigen
(immunology, metabolism)
- Diagnosis, Differential
- Female
- Humans
- Male
- Middle Aged
- Pleura
(metabolism, pathology)
- Pleural Effusion
(metabolism, pathology)
- Pleural Effusion, Malignant
(metabolism, pathology)
- Predictive Value of Tests
- V-Set Domain-Containing T-Cell Activation Inhibitor 1
(metabolism)
|