Abstract |
DNA sequences involved in the expression of the agropine synthase gene (ags) of T-DNA were identified by analysis of transcriptional activity of promoter mutants in crown gall tumors of sunflower. Precise quantification of activity was achieved using a homologous reference gene as an internal standard. Analysis of 5'-deletion mutants demonstrated the requirement of 314 base pairs of upstream DNA sequences for optimal activity. Five regions involved in transcriptional regulation were identified in the 5'-flanking sequences between positions -74 and -314. Four of these regions make a positive contribution to promoter activity, and the fifth exerts a negative influence. The TATA motif (-26 to -33) and the TATA proximal domain (-74 to -105), which contains two sequences similar to the mammalian CCAAT box, are the major determinants of promoter activity. The two TATA distal domains A and B are separated by a negative element (-166 to -205) which may attenuate promoter strength by distancing the TATA distal domain B (-206 to -314 base pairs) from downstream components of the promoter. The TATA distal domain B contains the a/b repeat first described in the nopaline synthase (nos) promoter and was unable to support transcription in the absence of elements within the TATA proximal domain.
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Authors | R S Bandyopadhyay, W B Bruce, W B Gurley |
Journal | The Journal of biological chemistry
(J Biol Chem)
Vol. 264
Issue 32
Pg. 19399-406
(Nov 15 1989)
ISSN: 0021-9258 [Print] United States |
PMID | 2808432
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Base Sequence
- Chromosome Deletion
- DNA, Bacterial
(genetics)
- Genes, Bacterial
- Molecular Sequence Data
- Mutation
- Plasmids
- Promoter Regions, Genetic
- Regulatory Sequences, Nucleic Acid
- Restriction Mapping
- Rhizobium
(enzymology, genetics)
- Transcription, Genetic
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