Abstract | BACKGROUND: METHODS: Using microarray analysis, the alterations of miRNA expression profiles were investigated in EGCG-treated CNE2 cells. Furthermore, the target genes and signaling pathways regulated by EGCG-specific miRNAs were identified using target prediction program and gene ontology analysis. RESULTS: A total of 14 miRNAs exhibited >2-fold expression changes in a dose-dependent manner after treatment with 20 μmol/L and 40 μmol/L EGCG. Totally 43, 49, and 52 target genes from these differentially expressed miRNAs were associated with the apoptosis, cell cycle regulation, and cell proliferation, respectively. A total of 66 signaling pathways, primarily involved in cancer development and lipid and glucose metabolism, were shown to be regulated by EGCG-specific miRNAs. CONCLUSION: EGCG induces considerable alterations of miRNA expression profiles in CNE2 cells, which provides mechanistic insights into cellular responses and antitumor activity mediated by EGCG.
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Authors | Bin-Bin Li, Guo-Liang Huang, Hua-Hui Li, Xia Kong, Zhi-Wei He |
Journal | Chinese medical journal
(Chin Med J (Engl))
Vol. 130
Issue 1
Pg. 93-99
(01 05 2017)
ISSN: 2542-5641 [Electronic] China |
PMID | 28051030
(Publication Type: Journal Article)
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Chemical References |
- Antineoplastic Agents
- MicroRNAs
- Catechin
- epigallocatechin gallate
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Topics |
- Antineoplastic Agents
(pharmacology)
- Carcinoma
- Catechin
(analogs & derivatives, pharmacology)
- Cell Line, Tumor
- Computational Biology
- Gene Expression
(drug effects, genetics)
- Humans
- MicroRNAs
(genetics, metabolism)
- Nasopharyngeal Carcinoma
- Nasopharyngeal Neoplasms
(genetics)
- Real-Time Polymerase Chain Reaction
- Signal Transduction
(drug effects, genetics)
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