Human alpha-L-fucosidase: complete coding sequence from cDNA clones.

Abstract

The human lysosomal storage disorder fucosidosis results from the deficiency of alpha-L-fucosidase, a lysosomal enzyme essential for the catabolism of oligosaccharides containing alpha-L-fucosides. cDNA clones coding for human alpha-L-fucosidase have been isolated from lambda gt10 and lambda gt11 cDNA libraries derived from human liver, placenta and colon. Compilation of cDNA sequences results in a nucleotide sequence of 2053 base pairs encoding alpha-L-fucosidase. The sequence contains an open reading frame of 461 amino acids beginning with the first in-frame methionine and includes 439 amino acids which comprise the mature protein in addition to a hydrophobic signal peptide sequence of 22 amino acids.

• Authors: T Occhiodoro, K R Beckmann, C P Morris, J J Hopwood
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 164 Issue 1 Pg. 439-45 (Oct 16 1989) ISSN: 0006-291X [Print] United States
• PMID: 2803312 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA Probes
• DNA
• alpha-L-Fucosidase

Topics

• Amino Acid Sequence
• Base Sequence
• DNA (genetics)
• DNA Probes
• Genomic Library
• Humans
• Molecular Sequence Data
• Restriction Mapping
• alpha-L-Fucosidase (genetics)