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[Alteration of leukocyte AMP- and IMP-pyrophosphorylase stability in leukemia].

Abstract
Effect of thermic treatment and of pre-incubation with phosphoribosyl pyrophosphate on activity of AMP- and IMP-pyrophosphorylases were studied in leukocytes under chronic forms of limpho- and myeloleukoses. AMP-pyrophosphorylase from leukemia leukocytes was inactivated after heating up to 65 degrees, which, at the same time, as distinct from the enzyme of normal leukocytes, proved to be reversibly reduced after incubation of the heated cell extracts with phosphoribosyl pyrophosphate. IMP-pyrophosphorylase of leukocytes was activated at this temperature; the activation of the enzyme was 15--20% higher in leukemic leukocytes than in leukocytes of healthy donors. The data obtained demonstrate the increased thermostability of purine nucleotide pyrophosphorylase from leukemic leukocytes, which is apparently due to conformational peculiarities of the enzyme molecules.
AuthorsV N Filippova, L I Filanovskaia, M N Blinov, E A Ushakova
JournalVoprosy meditsinskoi khimii (Vopr Med Khim) 1978 Sep-Oct Vol. 24 Issue 5 Pg. 666-70 ISSN: 0042-8809 [Print] Russia (Federation)
Vernacular TitleIzmenenie termostabil'nosti AMF- i IMF-pirofosforilazy leÄ­kotsitov pri leÄ­kozakh.
PMID280016 (Publication Type: English Abstract, Journal Article)
Chemical References
  • Pentosyltransferases
  • Adenine Phosphoribosyltransferase
  • Hypoxanthine Phosphoribosyltransferase
Topics
  • Adenine Phosphoribosyltransferase (metabolism)
  • Granulocytes (enzymology)
  • Hot Temperature
  • Humans
  • Hypoxanthine Phosphoribosyltransferase (metabolism)
  • Leukemia (blood, enzymology)
  • Leukemia, Lymphoid (enzymology)
  • Leukemia, Myeloid (enzymology)
  • Leukocytes (enzymology)
  • Lymphocytes (enzymology)
  • Pentosyltransferases (metabolism)

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