Abstract |
Repair kinetics of ultraviolet (UV) light-induced (6-4) photoproducts in xeroderma pigmentosum complementation group A, D, and variant cells were studied by the enzyme-linked immunosorbent assay (ELISA) using a specific monoclonal antibody raised against (6-4) photoproducts, together with unscheduled DNA synthesis (UDS) and loss of T4 endonuclease V-susceptible sites (ESS). Group AXP35KO cells completely failed to repair both ESS ( cyclobutane pyrimidine dimers) and antibody-recognizing (6-4) photoproducts until tested 24 h after irradiation, and had 2% early-time UDS. Group DXP43KO cells showed about 10% removal of both (6-4) photoproducts and ESS in 24 h, despite showing a residually higher level of 40% early-time and cumulative UDS. Thus, the results substantiated the extreme UV hypersensitivity of XP group A and D cells. However, XP52KO variant cells exhibited the normal level of UDS and ESS loss, but a slightly reduced repair of antibody-recognizing (6-4) photoproducts at 6 and 12 h after irradiation, which may account for a small UV hypersensitivity of the XP variant cells.
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Authors | T Hiramoto, T Matsunaga, M Ichihashi, O Nikaido, Y Fujiwara, Y Mishima |
Journal | The Journal of investigative dermatology
(J Invest Dermatol)
Vol. 93
Issue 5
Pg. 703-6
(Nov 1989)
ISSN: 0022-202X [Print] United States |
PMID | 2794553
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Monoclonal
- DNA
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Topics |
- Antibodies, Monoclonal
- DNA
(radiation effects)
- DNA Damage
- DNA Repair
- Dose-Response Relationship, Radiation
- Enzyme-Linked Immunosorbent Assay
- Humans
- In Vitro Techniques
- Kinetics
- Ultraviolet Rays
- Xeroderma Pigmentosum
(genetics, physiopathology)
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