The effect of CPH 82 on the growth of human lymphocytes in vitro. Definition of cytobiological action.

Abstract

A drug composed of two semisynthetic podophylline derivatives, CPH 82, has recently been launched for the treatment of severe rheumatoid arthritis. The present in vitro study of PHA-stimulated human T-lymphocytes showed that CPH 82 arrested cell division in a metaphase-like configuration. The cell cycle effects of CPH 82 were indistinguishable from the cell cycle effects of the classical microtubule depolymerizers, Colcemid (a colchicine derivative) and podophyllotoxin. A CPH 82 concentration of 1 microgram/ml, which is close to therapeutic serum concentrations, had an almost maximal effect on cell division. It is suggested that at least part of the anti-inflammatory effect of CPH 82 is due to a colchicine-like activity on, for example, proliferating lymphocytes.

• Authors: S Rantapää Dahlqvist, B Norberg, K Sondell, I Nordenson, G Holmgren
• Journal: British journal of rheumatology (Br J Rheumatol) Vol. 28 Issue 5 Pg. 418-21 (Oct 1989) ISSN: 0263-7103 [Print] England
• PMID: 2790402 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• CPH 82
• Glycosides
• Lignans
• Plant Extracts

Topics

• Glycosides (pharmacology)
• Humans
• Lignans
• Mitotic Index (drug effects)
• Plant Extracts (pharmacology)
• T-Lymphocytes (drug effects)